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Structure and organization of transposable mating type cassettes in Saccharomyces yeasts.
Proc Natl Acad Sci U S A. 1980 May; 77(5):2839-43.PN

Abstract

Cell type in Saccharomyces yeasts is regulated by two transposable blocks of DNA, the a and alpha cassettes. There are three loci where either cassette can exist. At the HML and HMR loci the cassettes are not expressed. The cassette at the MAT locus is expressed and controls the cell type. Changes of cell type involve transposition-substitution of cassettes from HML or HMR into MAT. We recently reported the molecular cloning of the alpha cassette at the HML locus, HML alpha, and showed that it contained sequences homologous to HMR and MAT. Using HML alpha as a hybridization probe, we have isolated HMLa, HMR alpha, HMRa, MAT alpha, and MATa. Heteroduplex analysis and restriction endonuclease mapping studies indicate that the a and alpha cassettes differ by a substitution corresponding to about 750 base pairs in alpha and about 600 base pairs in a. The HML, HMR, and MAT loci have regions of homology flanking the position of the a versus alpha substitution. We have used specific chromosome rearrangements fusing MAT and HML and MAT with HMR to orient the cloned sequences on the genetic map and have found that all three genes have the same left-to-right polarity on the chromosome.

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Authors

Strathern JN
No affiliation info available
Spatola E
No affiliation info available
McGill C
No affiliation info available
Hicks JB
No affiliation info available

MeSH

AllelesBase SequenceChromosome MappingCloning, MolecularDNA Transposable ElementsDNA, RecombinantRecombination, GeneticReproductionSaccharomyces cerevisiae

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6248870

Citation

Strathern, J N., et al. "Structure and Organization of Transposable Mating Type Cassettes in Saccharomyces Yeasts." Proceedings of the National Academy of Sciences of the United States of America, vol. 77, no. 5, 1980, pp. 2839-43.
Strathern JN, Spatola E, McGill C, et al. Structure and organization of transposable mating type cassettes in Saccharomyces yeasts. Proc Natl Acad Sci U S A. 1980;77(5):2839-43.
Strathern, J. N., Spatola, E., McGill, C., & Hicks, J. B. (1980). Structure and organization of transposable mating type cassettes in Saccharomyces yeasts. Proceedings of the National Academy of Sciences of the United States of America, 77(5), 2839-43.
Strathern JN, et al. Structure and Organization of Transposable Mating Type Cassettes in Saccharomyces Yeasts. Proc Natl Acad Sci U S A. 1980;77(5):2839-43. PubMed PMID: 6248870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structure and organization of transposable mating type cassettes in Saccharomyces yeasts. AU - Strathern,J N, AU - Spatola,E, AU - McGill,C, AU - Hicks,J B, PY - 1980/5/1/pubmed PY - 1980/5/1/medline PY - 1980/5/1/entrez SP - 2839 EP - 43 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 77 IS - 5 N2 - Cell type in Saccharomyces yeasts is regulated by two transposable blocks of DNA, the a and alpha cassettes. There are three loci where either cassette can exist. At the HML and HMR loci the cassettes are not expressed. The cassette at the MAT locus is expressed and controls the cell type. Changes of cell type involve transposition-substitution of cassettes from HML or HMR into MAT. We recently reported the molecular cloning of the alpha cassette at the HML locus, HML alpha, and showed that it contained sequences homologous to HMR and MAT. Using HML alpha as a hybridization probe, we have isolated HMLa, HMR alpha, HMRa, MAT alpha, and MATa. Heteroduplex analysis and restriction endonuclease mapping studies indicate that the a and alpha cassettes differ by a substitution corresponding to about 750 base pairs in alpha and about 600 base pairs in a. The HML, HMR, and MAT loci have regions of homology flanking the position of the a versus alpha substitution. We have used specific chromosome rearrangements fusing MAT and HML and MAT with HMR to orient the cloned sequences on the genetic map and have found that all three genes have the same left-to-right polarity on the chromosome. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/6248870/Structure_and_organization_of_transposable_mating_type_cassettes_in_Saccharomyces_yeasts_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/6248870/ DB - PRIME DP - Unbound Medicine ER -