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Regulation of coenzyme A biosynthesis by glucagon and glucocorticoid in adult rat liver parenchymal cells.
Biochem J. 1980 Apr 15; 188(1):175-84.BJ

Abstract

We studied the effects of glucagon, dibutyryl cyclic AMP and dexamethasone on the rate of [(14)C]pantothenate conversion to CoA in adult rat liver parenchymal cells in primary culture. The presence of 30nm-glucagon increased the rate by about 1.5-fold relative to control cultures (range 1.4-2.3) and 2.4-fold relative to cultures containing 1-3m-i.u. of insulin/ml. The half-maximal effect was obtained at 3nm-glucagon. Dibutyryl cyclic AMP plus theophylline also enhanced the rate by about 1.5-fold. Dexamethasone acted synergistically with glucagon; glucagon at 0.3nm had no effect when added alone, but resulted in a 1.7-fold enhancement when added in the presence of dexamethasone (maximum effect at 50nm). The 1.4-fold enhancement caused by the addition of saturating glucagon concentrations was increased to a 3-fold overall enhancement by the addition of dexamethasone. However, dexamethasone added alone over the range 5nm to 5mum had no effect on the rate of [(14)C]pantothenate conversion to CoA. The stimulatory effect of dibutyryl cyclic AMP plus theophylline was also enhanced by the addition of dexamethasone. Changes in intracellular pantothenate concentration or radioactivity could not account for the stimulatory effects of glucagon, dibutyryl cyclic AMP or dexamethasone. Addition of 18mum-cycloheximide, an inhibitor of protein synthesis, decreased the rate of incorporation of [(14)C]pantothenate into CoA and the enhancement of this rate by glucagon and dibutyryl cyclic AMP plus theophylline in a reversible manner. These results demonstrate an influence of glucagon, dibutyryl cyclic AMP and glucocorticoids on the intracellular mechanism regulating total CoA concentrations in the liver.

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Authors

Smith CM
No affiliation info available
Savage CR
No affiliation info available

MeSH

AnimalsBucladesineCells, CulturedCoenzyme ACycloheximideDexamethasoneGlucagonGlucocorticoidsInsulinLiverMalePantothenic AcidRatsTheophylline

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6250539

Citation

Smith, C M., and C R. Savage. "Regulation of Coenzyme a Biosynthesis By Glucagon and Glucocorticoid in Adult Rat Liver Parenchymal Cells." The Biochemical Journal, vol. 188, no. 1, 1980, pp. 175-84.
Smith CM, Savage CR. Regulation of coenzyme A biosynthesis by glucagon and glucocorticoid in adult rat liver parenchymal cells. Biochem J. 1980;188(1):175-84.
Smith, C. M., & Savage, C. R. (1980). Regulation of coenzyme A biosynthesis by glucagon and glucocorticoid in adult rat liver parenchymal cells. The Biochemical Journal, 188(1), 175-84.
Smith CM, Savage CR. Regulation of Coenzyme a Biosynthesis By Glucagon and Glucocorticoid in Adult Rat Liver Parenchymal Cells. Biochem J. 1980 Apr 15;188(1):175-84. PubMed PMID: 6250539.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of coenzyme A biosynthesis by glucagon and glucocorticoid in adult rat liver parenchymal cells. AU - Smith,C M, AU - Savage,C R,Jr PY - 1980/4/15/pubmed PY - 2001/3/28/medline PY - 1980/4/15/entrez SP - 175 EP - 84 JF - The Biochemical journal JO - Biochem J VL - 188 IS - 1 N2 - We studied the effects of glucagon, dibutyryl cyclic AMP and dexamethasone on the rate of [(14)C]pantothenate conversion to CoA in adult rat liver parenchymal cells in primary culture. The presence of 30nm-glucagon increased the rate by about 1.5-fold relative to control cultures (range 1.4-2.3) and 2.4-fold relative to cultures containing 1-3m-i.u. of insulin/ml. The half-maximal effect was obtained at 3nm-glucagon. Dibutyryl cyclic AMP plus theophylline also enhanced the rate by about 1.5-fold. Dexamethasone acted synergistically with glucagon; glucagon at 0.3nm had no effect when added alone, but resulted in a 1.7-fold enhancement when added in the presence of dexamethasone (maximum effect at 50nm). The 1.4-fold enhancement caused by the addition of saturating glucagon concentrations was increased to a 3-fold overall enhancement by the addition of dexamethasone. However, dexamethasone added alone over the range 5nm to 5mum had no effect on the rate of [(14)C]pantothenate conversion to CoA. The stimulatory effect of dibutyryl cyclic AMP plus theophylline was also enhanced by the addition of dexamethasone. Changes in intracellular pantothenate concentration or radioactivity could not account for the stimulatory effects of glucagon, dibutyryl cyclic AMP or dexamethasone. Addition of 18mum-cycloheximide, an inhibitor of protein synthesis, decreased the rate of incorporation of [(14)C]pantothenate into CoA and the enhancement of this rate by glucagon and dibutyryl cyclic AMP plus theophylline in a reversible manner. These results demonstrate an influence of glucagon, dibutyryl cyclic AMP and glucocorticoids on the intracellular mechanism regulating total CoA concentrations in the liver. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/6250539/Regulation_of_coenzyme_A_biosynthesis_by_glucagon_and_glucocorticoid_in_adult_rat_liver_parenchymal_cells_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/bj1880175 DB - PRIME DP - Unbound Medicine ER -