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Renal parathyroid hormone-dependent adenylate cyclase activity after repletion of vitamin D-deficient rats with vitamin D-2.
Biochim Biophys Acta. 1980 May 22; 629(3):546-52.BB

Abstract

Rats fed a diet deficient in both vitamin D and Ca2+ exhibited a greater depression of the renal parathyroid hormone (PTH)-dependent adenylate cyclase than was observed in rats fed diets deficient in either vitamin D or calcium. Total serum Ca2+ was decreased from a control level of 11.2 mg/dl to 8.5 mg/dl in rats fed the diet deficient in calcium alone, and to 5.4 mg/dl in rats fed the diet deficient in vitamin D. Serum calcium was decreased further to 4.3 mg/dl in rats fed the diet deficient in both vitamin D and Ca2+. Serum immuno-reactive PTH was significantly elevated over control levels when rats were fed the test diets; however, there were no significant differences between the elevated levels in the three experimental groups. Repletion of rats deficient in vitamin D only with a single oral dose of 3200 I.U. vitamin D-2 resulted in restoration of serum calcium to normal levels, a return of serum PTH to the control state, and an associated increase in PTH-dependent adenylate cyclase activity to the control level by 72 h. Repletion of rats deficient in both vitamin D and Ca2+ with the same dose of vitamin D-2 raised serum Ca2+ to 7.2 mg/dl by 72 h, but did not cause a reduction in circulating PTH, nor did it result in any significant improvement in the responsiveness of the membrane adenylate cyclase to PTH. These results suggest that elevated PTH is a factor in the down regulation of the PTH-dependent adenylate cyclase, but do not rule out a role for calcium as a regulatory factor.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6251904

Citation

Carnes, D L., et al. "Renal Parathyroid Hormone-dependent Adenylate Cyclase Activity After Repletion of Vitamin D-deficient Rats With Vitamin D-2." Biochimica Et Biophysica Acta, vol. 629, no. 3, 1980, pp. 546-52.
Carnes DL, Anast CS, Forte LR. Renal parathyroid hormone-dependent adenylate cyclase activity after repletion of vitamin D-deficient rats with vitamin D-2. Biochim Biophys Acta. 1980;629(3):546-52.
Carnes, D. L., Anast, C. S., & Forte, L. R. (1980). Renal parathyroid hormone-dependent adenylate cyclase activity after repletion of vitamin D-deficient rats with vitamin D-2. Biochimica Et Biophysica Acta, 629(3), 546-52.
Carnes DL, Anast CS, Forte LR. Renal Parathyroid Hormone-dependent Adenylate Cyclase Activity After Repletion of Vitamin D-deficient Rats With Vitamin D-2. Biochim Biophys Acta. 1980 May 22;629(3):546-52. PubMed PMID: 6251904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Renal parathyroid hormone-dependent adenylate cyclase activity after repletion of vitamin D-deficient rats with vitamin D-2. AU - Carnes,D L, AU - Anast,C S, AU - Forte,L R, PY - 1980/5/22/pubmed PY - 1980/5/22/medline PY - 1980/5/22/entrez SP - 546 EP - 52 JF - Biochimica et biophysica acta JO - Biochim. Biophys. Acta VL - 629 IS - 3 N2 - Rats fed a diet deficient in both vitamin D and Ca2+ exhibited a greater depression of the renal parathyroid hormone (PTH)-dependent adenylate cyclase than was observed in rats fed diets deficient in either vitamin D or calcium. Total serum Ca2+ was decreased from a control level of 11.2 mg/dl to 8.5 mg/dl in rats fed the diet deficient in calcium alone, and to 5.4 mg/dl in rats fed the diet deficient in vitamin D. Serum calcium was decreased further to 4.3 mg/dl in rats fed the diet deficient in both vitamin D and Ca2+. Serum immuno-reactive PTH was significantly elevated over control levels when rats were fed the test diets; however, there were no significant differences between the elevated levels in the three experimental groups. Repletion of rats deficient in vitamin D only with a single oral dose of 3200 I.U. vitamin D-2 resulted in restoration of serum calcium to normal levels, a return of serum PTH to the control state, and an associated increase in PTH-dependent adenylate cyclase activity to the control level by 72 h. Repletion of rats deficient in both vitamin D and Ca2+ with the same dose of vitamin D-2 raised serum Ca2+ to 7.2 mg/dl by 72 h, but did not cause a reduction in circulating PTH, nor did it result in any significant improvement in the responsiveness of the membrane adenylate cyclase to PTH. These results suggest that elevated PTH is a factor in the down regulation of the PTH-dependent adenylate cyclase, but do not rule out a role for calcium as a regulatory factor. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/6251904/Renal_parathyroid_hormone_dependent_adenylate_cyclase_activity_after_repletion_of_vitamin_D_deficient_rats_with_vitamin_D_2_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0304-4165(80)90160-9 DB - PRIME DP - Unbound Medicine ER -