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Selection and characterization of ColE1 plasmid mutants that exhibit altered stability and replication.
J Bacteriol. 1981 Sep; 147(3):962-71.JB

Abstract

This report describes a method for isolating mutants of plasmid ColE1 that exhibit unstable maintenance and altered replication characteristics. It also describes the initial characterization of four mutants isolated by that method. A chimeric plasmid, pHSG124, containing a ColE1 derivative and a temperature-sensitive replication derivative of pSC101 was mutagenized in vitro, using hydroxylamine. By adjusting the growth conditions of transformants containing the mutagenized chimeric deoxyribonucleic acid, it was possible to rapidly screen colonies and identify those that had a high probability of carrying ColE1 mutants that exhibit unstable maintenance. Of those mutants, some exhibited altered copy number or accumulated catenated structures. Evidence is presented which suggests that the mutations in three of the mutants are probably located in the HaeII A fragment of ColE1.

Links

Publisher Full Text
ncbi.nlm.nih.gov
jb.asm.org
PMC Free PDF

Authors

Inselburg J
No affiliation info available

MeSH

Bacteriocin PlasmidsChloramphenicolDNA ReplicationDNA Restriction EnzymesDeoxyribonucleases, Type II Site-SpecificEscherichia coliMutationNucleic Acid ConformationNucleic Acid HybridizationPlasmidsTemperatureTransformation, Bacterial

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6268615

Citation

Inselburg, J. "Selection and Characterization of ColE1 Plasmid Mutants That Exhibit Altered Stability and Replication." Journal of Bacteriology, vol. 147, no. 3, 1981, pp. 962-71.
Inselburg J. Selection and characterization of ColE1 plasmid mutants that exhibit altered stability and replication. J Bacteriol. 1981;147(3):962-71.
Inselburg, J. (1981). Selection and characterization of ColE1 plasmid mutants that exhibit altered stability and replication. Journal of Bacteriology, 147(3), 962-71.
Inselburg J. Selection and Characterization of ColE1 Plasmid Mutants That Exhibit Altered Stability and Replication. J Bacteriol. 1981;147(3):962-71. PubMed PMID: 6268615.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selection and characterization of ColE1 plasmid mutants that exhibit altered stability and replication. A1 - Inselburg,J, PY - 1981/9/1/pubmed PY - 1981/9/1/medline PY - 1981/9/1/entrez SP - 962 EP - 71 JF - Journal of bacteriology JO - J Bacteriol VL - 147 IS - 3 N2 - This report describes a method for isolating mutants of plasmid ColE1 that exhibit unstable maintenance and altered replication characteristics. It also describes the initial characterization of four mutants isolated by that method. A chimeric plasmid, pHSG124, containing a ColE1 derivative and a temperature-sensitive replication derivative of pSC101 was mutagenized in vitro, using hydroxylamine. By adjusting the growth conditions of transformants containing the mutagenized chimeric deoxyribonucleic acid, it was possible to rapidly screen colonies and identify those that had a high probability of carrying ColE1 mutants that exhibit unstable maintenance. Of those mutants, some exhibited altered copy number or accumulated catenated structures. Evidence is presented which suggests that the mutations in three of the mutants are probably located in the HaeII A fragment of ColE1. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/6268615/Selection_and_characterization_of_ColE1_plasmid_mutants_that_exhibit_altered_stability_and_replication_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=6268615 DB - PRIME DP - Unbound Medicine ER -