Abstract
This report describes a method for isolating mutants of plasmid ColE1 that exhibit unstable maintenance and altered replication characteristics. It also describes the initial characterization of four mutants isolated by that method. A chimeric plasmid, pHSG124, containing a ColE1 derivative and a temperature-sensitive replication derivative of pSC101 was mutagenized in vitro, using hydroxylamine. By adjusting the growth conditions of transformants containing the mutagenized chimeric deoxyribonucleic acid, it was possible to rapidly screen colonies and identify those that had a high probability of carrying ColE1 mutants that exhibit unstable maintenance. Of those mutants, some exhibited altered copy number or accumulated catenated structures. Evidence is presented which suggests that the mutations in three of the mutants are probably located in the HaeII A fragment of ColE1.
TY - JOUR
T1 - Selection and characterization of ColE1 plasmid mutants that exhibit altered stability and replication.
A1 - Inselburg,J,
PY - 1981/9/1/pubmed
PY - 1981/9/1/medline
PY - 1981/9/1/entrez
SP - 962
EP - 71
JF - Journal of bacteriology
JO - J Bacteriol
VL - 147
IS - 3
N2 - This report describes a method for isolating mutants of plasmid ColE1 that exhibit unstable maintenance and altered replication characteristics. It also describes the initial characterization of four mutants isolated by that method. A chimeric plasmid, pHSG124, containing a ColE1 derivative and a temperature-sensitive replication derivative of pSC101 was mutagenized in vitro, using hydroxylamine. By adjusting the growth conditions of transformants containing the mutagenized chimeric deoxyribonucleic acid, it was possible to rapidly screen colonies and identify those that had a high probability of carrying ColE1 mutants that exhibit unstable maintenance. Of those mutants, some exhibited altered copy number or accumulated catenated structures. Evidence is presented which suggests that the mutations in three of the mutants are probably located in the HaeII A fragment of ColE1.
SN - 0021-9193
UR - https://www.unboundmedicine.com/medline/citation/6268615/Selection_and_characterization_of_ColE1_plasmid_mutants_that_exhibit_altered_stability_and_replication_
L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=6268615
DB - PRIME
DP - Unbound Medicine
ER -