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ColE1 copy number mutants.
J Bacteriol. 1982 Aug; 151(2):845-54.JB

Abstract

A deletion mutant of the colicin E1-derived plasmid, pDMS6642, exhibited an approximately fourfold increase in copy number. We subsequently isolated hydroxylamine-induced mutants of that plasmid that had a further increase in copy number. Analysis of them suggests that the increased copy number of pDMS6642 is associated with transcriptional readthrough from a Tn3 transposon into the region of ColE1 containing information that influences plasmid replication. The hydroxylamine mutation in one copy number mutant appeared to increase the plasmid copy number by stimulating readthrough transcription from the Tn3 transposon into the ColE1 replication control region, whereas the other hydroxylamine mutation acts by another mechanism.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6284714

Citation

Schmidt, L, and J Inselburg. "ColE1 Copy Number Mutants." Journal of Bacteriology, vol. 151, no. 2, 1982, pp. 845-54.
Schmidt L, Inselburg J. ColE1 copy number mutants. J Bacteriol. 1982;151(2):845-54.
Schmidt, L., & Inselburg, J. (1982). ColE1 copy number mutants. Journal of Bacteriology, 151(2), 845-54.
Schmidt L, Inselburg J. ColE1 Copy Number Mutants. J Bacteriol. 1982;151(2):845-54. PubMed PMID: 6284714.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ColE1 copy number mutants. AU - Schmidt,L, AU - Inselburg,J, PY - 1982/8/1/pubmed PY - 1982/8/1/medline PY - 1982/8/1/entrez SP - 845 EP - 54 JF - Journal of bacteriology JO - J. Bacteriol. VL - 151 IS - 2 N2 - A deletion mutant of the colicin E1-derived plasmid, pDMS6642, exhibited an approximately fourfold increase in copy number. We subsequently isolated hydroxylamine-induced mutants of that plasmid that had a further increase in copy number. Analysis of them suggests that the increased copy number of pDMS6642 is associated with transcriptional readthrough from a Tn3 transposon into the region of ColE1 containing information that influences plasmid replication. The hydroxylamine mutation in one copy number mutant appeared to increase the plasmid copy number by stimulating readthrough transcription from the Tn3 transposon into the ColE1 replication control region, whereas the other hydroxylamine mutation acts by another mechanism. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/6284714/ColE1_copy_number_mutants_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=6284714 DB - PRIME DP - Unbound Medicine ER -