Tags

Type your tag names separated by a space and hit enter

Genetic map of the crown gall suppressive IncW plasmid pSa.
Mol Gen Genet 1982; 186(1):10-5MG

Abstract

A genetic map of the W incompatibility group plasmid pSa has been prepared through the construction of deletion derivatives of pSa and the cloning of various fragments of pSa in pBR322. Phenotypic analysis of these derivatives has identified the location of genes encoding resistance to chloramphenicol, sulfonamides, spectinomycin, streptomycin, kanamycin, gentamycin, and tobramycin. Information sufficient for the replication of the plasmid in both Escherichia coli and Agrobacterium tumefaciens is contained within a 4 kilobase pair region. Two regions have been identified as involved in the transfer of the plasmid; one of these regions is also involved in the inhibition of oncogenesis by pSa when it is present in an oncogenic strain of A. tumefaciens. Certain of the deletion derivatives of pSa are potential vectors for the cloning and analysis of A. tumefaciens Ti plasmid DNA.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6287164

Citation

Tait, R C., et al. "Genetic Map of the Crown Gall Suppressive IncW Plasmid PSa." Molecular & General Genetics : MGG, vol. 186, no. 1, 1982, pp. 10-5.
Tait RC, Lundquist RC, Kado CI. Genetic map of the crown gall suppressive IncW plasmid pSa. Mol Gen Genet. 1982;186(1):10-5.
Tait, R. C., Lundquist, R. C., & Kado, C. I. (1982). Genetic map of the crown gall suppressive IncW plasmid pSa. Molecular & General Genetics : MGG, 186(1), pp. 10-5.
Tait RC, Lundquist RC, Kado CI. Genetic Map of the Crown Gall Suppressive IncW Plasmid PSa. Mol Gen Genet. 1982;186(1):10-5. PubMed PMID: 6287164.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic map of the crown gall suppressive IncW plasmid pSa. AU - Tait,R C, AU - Lundquist,R C, AU - Kado,C I, PY - 1982/1/1/pubmed PY - 1982/1/1/medline PY - 1982/1/1/entrez SP - 10 EP - 5 JF - Molecular & general genetics : MGG JO - Mol. Gen. Genet. VL - 186 IS - 1 N2 - A genetic map of the W incompatibility group plasmid pSa has been prepared through the construction of deletion derivatives of pSa and the cloning of various fragments of pSa in pBR322. Phenotypic analysis of these derivatives has identified the location of genes encoding resistance to chloramphenicol, sulfonamides, spectinomycin, streptomycin, kanamycin, gentamycin, and tobramycin. Information sufficient for the replication of the plasmid in both Escherichia coli and Agrobacterium tumefaciens is contained within a 4 kilobase pair region. Two regions have been identified as involved in the transfer of the plasmid; one of these regions is also involved in the inhibition of oncogenesis by pSa when it is present in an oncogenic strain of A. tumefaciens. Certain of the deletion derivatives of pSa are potential vectors for the cloning and analysis of A. tumefaciens Ti plasmid DNA. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/6287164/Genetic_map_of_the_crown_gall_suppressive_IncW_plasmid_pSa_ L2 - https://www.lens.org/lens/search?q=citation_id:6287164 DB - PRIME DP - Unbound Medicine ER -