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Effect of phenolphthalein on monkey intestinal water and electrolyte transport.
Am J Physiol. 1982 Oct; 243(4):G268-75.AJ

Abstract

To assess Na-K-ATPase inhibiton and prostaglandin synthesis stimulation as the mechanism of the secretory (cathartic) action of phenolphthalein in the primate, we investigated water and electrolyte transport and Na-K-ATPase levels in monkey intestine. Both jejunum and colon were studied with in vivo perfusion and in vitro Ussing chamber techniques. Water, Na, and Cl absorption was inhibited or secretion was induced by phenolphthalein (10(-3) M) in the jejunum and colon when the drug was present in the mucosal bathing (perfusion) solution. Serosal addition of phenolphthalein (10(-4) or 10(-3) M) induced Na and anion absorption in the jejunum but not in the colon. Phenolphthalein inhibited Na-K-ATPase activity in the test tube, but assays of intestine previously perfused or bathed in the drug showed no inhibiton. Indomethacin, in doses sufficient to inhibit prostaglandin synthesis in the intestine, inhibited the secretion induced by phenolphthalein in the jejunum but not in the colon. These inconsistencies cast doubt on the role of Na-K-ATPase inhibition or the role of prostaglandin synthesis stimulation in the mechanism of action of phenolphthalein.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6289679

Citation

Powell, D W., et al. "Effect of Phenolphthalein On Monkey Intestinal Water and Electrolyte Transport." The American Journal of Physiology, vol. 243, no. 4, 1982, pp. G268-75.
Powell DW, Johnson PT, Bryson JC, et al. Effect of phenolphthalein on monkey intestinal water and electrolyte transport. Am J Physiol. 1982;243(4):G268-75.
Powell, D. W., Johnson, P. T., Bryson, J. C., Orlando, R. C., & Fan, C. C. (1982). Effect of phenolphthalein on monkey intestinal water and electrolyte transport. The American Journal of Physiology, 243(4), G268-75.
Powell DW, et al. Effect of Phenolphthalein On Monkey Intestinal Water and Electrolyte Transport. Am J Physiol. 1982;243(4):G268-75. PubMed PMID: 6289679.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of phenolphthalein on monkey intestinal water and electrolyte transport. AU - Powell,D W, AU - Johnson,P T, AU - Bryson,J C, AU - Orlando,R C, AU - Fan,C C, PY - 1982/10/1/pubmed PY - 1982/10/1/medline PY - 1982/10/1/entrez SP - G268 EP - 75 JF - The American journal of physiology JO - Am. J. Physiol. VL - 243 IS - 4 N2 - To assess Na-K-ATPase inhibiton and prostaglandin synthesis stimulation as the mechanism of the secretory (cathartic) action of phenolphthalein in the primate, we investigated water and electrolyte transport and Na-K-ATPase levels in monkey intestine. Both jejunum and colon were studied with in vivo perfusion and in vitro Ussing chamber techniques. Water, Na, and Cl absorption was inhibited or secretion was induced by phenolphthalein (10(-3) M) in the jejunum and colon when the drug was present in the mucosal bathing (perfusion) solution. Serosal addition of phenolphthalein (10(-4) or 10(-3) M) induced Na and anion absorption in the jejunum but not in the colon. Phenolphthalein inhibited Na-K-ATPase activity in the test tube, but assays of intestine previously perfused or bathed in the drug showed no inhibiton. Indomethacin, in doses sufficient to inhibit prostaglandin synthesis in the intestine, inhibited the secretion induced by phenolphthalein in the jejunum but not in the colon. These inconsistencies cast doubt on the role of Na-K-ATPase inhibition or the role of prostaglandin synthesis stimulation in the mechanism of action of phenolphthalein. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/6289679/Effect_of_phenolphthalein_on_monkey_intestinal_water_and_electrolyte_transport_ L2 - http://journals.physiology.org/doi/full/10.1152/ajpgi.1982.243.4.G268?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -