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Visible-light-induced OH radicals in DNA-proflavine complexes: an e.p.r. and spin trapping study.

Abstract

Using the spin-trapping technique, irradiation with visible light of complexes between DNA and proflavine was shown to generate OH radicals. The characteristic spectra were not obtained when proflavine or DNA were irradiated alone, nor when oxygen was absent. Using DMPO as a spin trap we found that the intensity of the DMPO-OH e.p.r. signal was enhanced when the molar ratio between bound proflavine and the DNA phosphorus increased up to a value of 0.15 demonstrating the efficiency of the intercalated dye molecules. A strong decrease of the e.p.r. signal was observed in the presence of various OH. scavengers like t-butanol, isopropanol or sodium benzoate. The OH. production was also decreased when the irradiation was made in the presence of SOD, ceruloplasmin or catalase and after addition of Chelex 100 resin.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6290410

Citation

Piette, J, et al. "Visible-light-induced OH Radicals in DNA-proflavine Complexes: an E.p.r. and Spin Trapping Study." International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine, vol. 42, no. 2, 1982, pp. 151-61.
Piette J, Decuyper J, Machiroux R, et al. Visible-light-induced OH radicals in DNA-proflavine complexes: an e.p.r. and spin trapping study. Int J Radiat Biol Relat Stud Phys Chem Med. 1982;42(2):151-61.
Piette, J., Decuyper, J., Machiroux, R., Calberg-Bacq, C. M., Van de Vorst, A., & Lion, Y. (1982). Visible-light-induced OH radicals in DNA-proflavine complexes: an e.p.r. and spin trapping study. International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine, 42(2), 151-61.
Piette J, et al. Visible-light-induced OH Radicals in DNA-proflavine Complexes: an E.p.r. and Spin Trapping Study. Int J Radiat Biol Relat Stud Phys Chem Med. 1982;42(2):151-61. PubMed PMID: 6290410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Visible-light-induced OH radicals in DNA-proflavine complexes: an e.p.r. and spin trapping study. AU - Piette,J, AU - Decuyper,J, AU - Machiroux,R, AU - Calberg-Bacq,C M, AU - Van de Vorst,A, AU - Lion,Y, PY - 1982/8/1/pubmed PY - 1982/8/1/medline PY - 1982/8/1/entrez SP - 151 EP - 61 JF - International journal of radiation biology and related studies in physics, chemistry, and medicine JO - Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. VL - 42 IS - 2 N2 - Using the spin-trapping technique, irradiation with visible light of complexes between DNA and proflavine was shown to generate OH radicals. The characteristic spectra were not obtained when proflavine or DNA were irradiated alone, nor when oxygen was absent. Using DMPO as a spin trap we found that the intensity of the DMPO-OH e.p.r. signal was enhanced when the molar ratio between bound proflavine and the DNA phosphorus increased up to a value of 0.15 demonstrating the efficiency of the intercalated dye molecules. A strong decrease of the e.p.r. signal was observed in the presence of various OH. scavengers like t-butanol, isopropanol or sodium benzoate. The OH. production was also decreased when the irradiation was made in the presence of SOD, ceruloplasmin or catalase and after addition of Chelex 100 resin. SN - 0020-7616 UR - https://www.unboundmedicine.com/medline/citation/6290410/Visible_light_induced_OH_radicals_in_DNA_proflavine_complexes:_an_e_p_r__and_spin_trapping_study_ L2 - https://www.lens.org/lens/search?q=citation_id:6290410 DB - PRIME DP - Unbound Medicine ER -