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[Analysis of chromosomal and extrachromosomal functions involved in the replication of bacterial plasmids].
Rev Esp Fisiol. 1982; 38 Suppl:259-69.RE

Abstract

A variety of functions codified by both the host chromosome and the plasmid genome are necessary for plasmid replication. We have found that dna B and dna F are essential for replication of both types of plasmids: R144 and Sa which have a restricted control of replication, and R6K with a relaxed control; on the other hand plasmids belonging to the first group absolutely depend on dna A and dna E gene products. Plasmid Sa, which shares with the 1st group a restricted control of replication but appears on a number of intermediate copies, shows a partial requirement to the dna A gene product. Location studies of plasmid-coded genes, involved in the replications of plasmids, were carried out in plasmid pSC102, a derivative of the resistance plasmic R6-5. All the genes required for replication are contained into a DNA segment which consists of two Pst I fragments: P-4 and P-6. The origin of replication On V is not itself sufficient for autonomous replication but needs in addition the presence of the Rep A gene. Genes responsible for copy control and incompatibility Cop/Inc are located on fragment P-6, i.e. they are physically distinct from Ori V. This indicates that the origin of replication is not responsible for, although may participate in, the incompatibility phenomenon.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

spa

PubMed ID

6293016

Citation

Ortiz-Melón, J M., and I Andrés. "[Analysis of Chromosomal and Extrachromosomal Functions Involved in the Replication of Bacterial Plasmids]." Revista Espanola De Fisiologia, vol. 38 Suppl, 1982, pp. 259-69.
Ortiz-Melón JM, Andrés I. [Analysis of chromosomal and extrachromosomal functions involved in the replication of bacterial plasmids]. Rev Esp Fisiol. 1982;38 Suppl:259-69.
Ortiz-Melón, J. M., & Andrés, I. (1982). [Analysis of chromosomal and extrachromosomal functions involved in the replication of bacterial plasmids]. Revista Espanola De Fisiologia, 38 Suppl, 259-69.
Ortiz-Melón JM, Andrés I. [Analysis of Chromosomal and Extrachromosomal Functions Involved in the Replication of Bacterial Plasmids]. Rev Esp Fisiol. 1982;38 Suppl:259-69. PubMed PMID: 6293016.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Analysis of chromosomal and extrachromosomal functions involved in the replication of bacterial plasmids]. AU - Ortiz-Melón,J M, AU - Andrés,I, PY - 1982/1/1/pubmed PY - 1982/1/1/medline PY - 1982/1/1/entrez SP - 259 EP - 69 JF - Revista espanola de fisiologia JO - Rev. Esp. Fisiol. VL - 38 Suppl N2 - A variety of functions codified by both the host chromosome and the plasmid genome are necessary for plasmid replication. We have found that dna B and dna F are essential for replication of both types of plasmids: R144 and Sa which have a restricted control of replication, and R6K with a relaxed control; on the other hand plasmids belonging to the first group absolutely depend on dna A and dna E gene products. Plasmid Sa, which shares with the 1st group a restricted control of replication but appears on a number of intermediate copies, shows a partial requirement to the dna A gene product. Location studies of plasmid-coded genes, involved in the replications of plasmids, were carried out in plasmid pSC102, a derivative of the resistance plasmic R6-5. All the genes required for replication are contained into a DNA segment which consists of two Pst I fragments: P-4 and P-6. The origin of replication On V is not itself sufficient for autonomous replication but needs in addition the presence of the Rep A gene. Genes responsible for copy control and incompatibility Cop/Inc are located on fragment P-6, i.e. they are physically distinct from Ori V. This indicates that the origin of replication is not responsible for, although may participate in, the incompatibility phenomenon. SN - 0034-9402 UR - https://www.unboundmedicine.com/medline/citation/6293016/[Analysis_of_chromosomal_and_extrachromosomal_functions_involved_in_the_replication_of_bacterial_plasmids]_ DB - PRIME DP - Unbound Medicine ER -