Tags

Type your tag names separated by a space and hit enter

[Comparative analysis of the P-1-group plasmids of incompatibility].
Genetika. 1982 Oct; 18(10):1636-44.G

Abstract

Wide host range plasmids (IncP-1) R906, R751 and R702 have several cleavage sites for BamHI, HindIII and EcoRI enzymes, in contrast to RP4 plasmid. Using these enzymes, deletion mutants of R906 plasmid have been obtained in vitro which only lost short DNA fragments (1 to 14 kb). A narrow host range pAV1 plasmid of the same incompatibility group has been transformed into the cells of Escherichia coli. pAV1 is stably maintained in the new host and retains its narrow host range in the course of conjugation. Different restriction fragments of R702, R751, R906 and R906-derived deletion mutants hybridize with the nick-translated probe of RP4 DNA. It is suggested that the wide host range plasmids have a similarity in structural and functional organization.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

rus

PubMed ID

6293921

Citation

Azarian, N G., et al. "[Comparative Analysis of the P-1-group Plasmids of Incompatibility]." Genetika, vol. 18, no. 10, 1982, pp. 1636-44.
Azarian NG, Sakanian VA, Krupenko MA, et al. [Comparative analysis of the P-1-group plasmids of incompatibility]. Genetika. 1982;18(10):1636-44.
Azarian, N. G., Sakanian, V. A., Krupenko, M. A., & Alikhanian, S. I. (1982). [Comparative analysis of the P-1-group plasmids of incompatibility]. Genetika, 18(10), 1636-44.
Azarian NG, et al. [Comparative Analysis of the P-1-group Plasmids of Incompatibility]. Genetika. 1982;18(10):1636-44. PubMed PMID: 6293921.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparative analysis of the P-1-group plasmids of incompatibility]. AU - Azarian,N G, AU - Sakanian,V A, AU - Krupenko,M A, AU - Alikhanian,S I, PY - 1982/10/1/pubmed PY - 1982/10/1/medline PY - 1982/10/1/entrez SP - 1636 EP - 44 JF - Genetika JO - Genetika VL - 18 IS - 10 N2 - Wide host range plasmids (IncP-1) R906, R751 and R702 have several cleavage sites for BamHI, HindIII and EcoRI enzymes, in contrast to RP4 plasmid. Using these enzymes, deletion mutants of R906 plasmid have been obtained in vitro which only lost short DNA fragments (1 to 14 kb). A narrow host range pAV1 plasmid of the same incompatibility group has been transformed into the cells of Escherichia coli. pAV1 is stably maintained in the new host and retains its narrow host range in the course of conjugation. Different restriction fragments of R702, R751, R906 and R906-derived deletion mutants hybridize with the nick-translated probe of RP4 DNA. It is suggested that the wide host range plasmids have a similarity in structural and functional organization. SN - 0016-6758 UR - https://www.unboundmedicine.com/medline/citation/6293921/[Comparative_analysis_of_the_P_1_group_plasmids_of_incompatibility]_ DB - PRIME DP - Unbound Medicine ER -