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Spontaneous oxygen radical generation by sickle erythrocytes.
J Clin Invest 1982; 70(6):1253-9JCI

Abstract

Since the various membrane abnormalities of sickle erythrocytes might result from excessive accumulation of oxidant damage, we have measured the generation of superoxide, peroxide, and hydroxyl radical by normal and sickle erythrocytes using assays involving reduction of cytochrome c, aminotriazole inhibition of catalase, and methane evolution from dimethyl sulfoxide, respectively. Compared with normal erythrocytes, sickle erythrocytes spontaneously generate approximately twice as much superoxide, peroxide, and hydroxyl radical. One possible source of hydroxyl radical generation was identified as hemichrome, excessive amounts of which are bound to sickle erythrocyte membranes. Hemichrome did not generate hydroxyl radical when exposed to superoxide alone or peroxide alone. However, in the presence of both superoxide and peroxide, hemichrome greatly facilitated hydroxyl radical generation. Supporting this, normal erythrocyte membranes induced to acquire sickle hemichrome concomitantly acquired an enhanced ability to mediate hydroxyl radical generation. Finally, sickle erythrocyte membranes greatly enhanced superoxide/peroxide-driven hydroxyl radical generation as compared with normal erythrocyte membranes. These data suggest that an excessive accumulation of oxidant damage in sickle erythrocyte membranes might contribute to the accelerated membrane senescence of these cells. They further indicate that accumulation of oxidant damage could be a determinant of normal erythrocyte membrane senescence.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6294138

Citation

Hebbel, R P., et al. "Spontaneous Oxygen Radical Generation By Sickle Erythrocytes." The Journal of Clinical Investigation, vol. 70, no. 6, 1982, pp. 1253-9.
Hebbel RP, Eaton JW, Balasingam M, et al. Spontaneous oxygen radical generation by sickle erythrocytes. J Clin Invest. 1982;70(6):1253-9.
Hebbel, R. P., Eaton, J. W., Balasingam, M., & Steinberg, M. H. (1982). Spontaneous oxygen radical generation by sickle erythrocytes. The Journal of Clinical Investigation, 70(6), pp. 1253-9.
Hebbel RP, et al. Spontaneous Oxygen Radical Generation By Sickle Erythrocytes. J Clin Invest. 1982;70(6):1253-9. PubMed PMID: 6294138.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spontaneous oxygen radical generation by sickle erythrocytes. AU - Hebbel,R P, AU - Eaton,J W, AU - Balasingam,M, AU - Steinberg,M H, PY - 1982/12/1/pubmed PY - 1982/12/1/medline PY - 1982/12/1/entrez SP - 1253 EP - 9 JF - The Journal of clinical investigation JO - J. Clin. Invest. VL - 70 IS - 6 N2 - Since the various membrane abnormalities of sickle erythrocytes might result from excessive accumulation of oxidant damage, we have measured the generation of superoxide, peroxide, and hydroxyl radical by normal and sickle erythrocytes using assays involving reduction of cytochrome c, aminotriazole inhibition of catalase, and methane evolution from dimethyl sulfoxide, respectively. Compared with normal erythrocytes, sickle erythrocytes spontaneously generate approximately twice as much superoxide, peroxide, and hydroxyl radical. One possible source of hydroxyl radical generation was identified as hemichrome, excessive amounts of which are bound to sickle erythrocyte membranes. Hemichrome did not generate hydroxyl radical when exposed to superoxide alone or peroxide alone. However, in the presence of both superoxide and peroxide, hemichrome greatly facilitated hydroxyl radical generation. Supporting this, normal erythrocyte membranes induced to acquire sickle hemichrome concomitantly acquired an enhanced ability to mediate hydroxyl radical generation. Finally, sickle erythrocyte membranes greatly enhanced superoxide/peroxide-driven hydroxyl radical generation as compared with normal erythrocyte membranes. These data suggest that an excessive accumulation of oxidant damage in sickle erythrocyte membranes might contribute to the accelerated membrane senescence of these cells. They further indicate that accumulation of oxidant damage could be a determinant of normal erythrocyte membrane senescence. SN - 0021-9738 UR - https://www.unboundmedicine.com/medline/citation/6294138/Spontaneous_oxygen_radical_generation_by_sickle_erythrocytes_ L2 - https://doi.org/10.1172/jci110724 DB - PRIME DP - Unbound Medicine ER -