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Regulation of bacterial glycogen synthesis. Stimulation of glycogen synthesis by endogenous and exogenous cyclic adenosine 3':5'-monophosphate in Escherichia coli and the requirement for a functional CRP gene.
J Biol Chem. 1983 Mar 25; 258(6):3813-24.JB

Abstract

In Escherichia coli cya mutants, deficient in adenylate cyclase (EC 4.6.1.1), basal cellular rates of glycogen synthesis were lower and the relative increases produced by exogenous cyclic adenosine 3',5'-monophosphate during growth on glucose were greater than in their respective parent strains. These observations provide strong evidence that endogenous cyclic AMP is one of the key regulators of glycogen synthesis in growing E. coli. In crp mutants, deficient in cyclic AMP receptor protein (CRP), the basal cellular rates of glycogen synthesis were much lower than in their respective parent strains. Stimulation of glycogen synthesis by exogenous cyclic AMP was markedly attenuated in the three crp mutants. Thus, stimulation of glycogen synthesis by either endogenous or exogenous cyclic AMP appears to require CRP. Functional CRP appeared to be required for all three responses observed after cyclic AMP addition: an abrupt step-up in the cellular rate of glycogen synthesis, a continuing exponential increase in rate, and a stimulation of the rate during a subsequent nitrogen starvation. To account for these responses, we derived a mathematical model in which the cyclic AMP-CRP complex regulates the differential rate of synthesis of an enzyme metabolizing an effector of the rate-limiting enzyme of glycogen synthesis.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6300058

Citation

Leckie, M P., et al. "Regulation of Bacterial Glycogen Synthesis. Stimulation of Glycogen Synthesis By Endogenous and Exogenous Cyclic Adenosine 3':5'-monophosphate in Escherichia Coli and the Requirement for a Functional CRP Gene." The Journal of Biological Chemistry, vol. 258, no. 6, 1983, pp. 3813-24.
Leckie MP, Ng RH, Porter SE, et al. Regulation of bacterial glycogen synthesis. Stimulation of glycogen synthesis by endogenous and exogenous cyclic adenosine 3':5'-monophosphate in Escherichia coli and the requirement for a functional CRP gene. J Biol Chem. 1983;258(6):3813-24.
Leckie, M. P., Ng, R. H., Porter, S. E., Compton, D. R., & Dietzler, D. N. (1983). Regulation of bacterial glycogen synthesis. Stimulation of glycogen synthesis by endogenous and exogenous cyclic adenosine 3':5'-monophosphate in Escherichia coli and the requirement for a functional CRP gene. The Journal of Biological Chemistry, 258(6), 3813-24.
Leckie MP, et al. Regulation of Bacterial Glycogen Synthesis. Stimulation of Glycogen Synthesis By Endogenous and Exogenous Cyclic Adenosine 3':5'-monophosphate in Escherichia Coli and the Requirement for a Functional CRP Gene. J Biol Chem. 1983 Mar 25;258(6):3813-24. PubMed PMID: 6300058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of bacterial glycogen synthesis. Stimulation of glycogen synthesis by endogenous and exogenous cyclic adenosine 3':5'-monophosphate in Escherichia coli and the requirement for a functional CRP gene. AU - Leckie,M P, AU - Ng,R H, AU - Porter,S E, AU - Compton,D R, AU - Dietzler,D N, PY - 1983/3/25/pubmed PY - 1983/3/25/medline PY - 1983/3/25/entrez SP - 3813 EP - 24 JF - The Journal of biological chemistry JO - J Biol Chem VL - 258 IS - 6 N2 - In Escherichia coli cya mutants, deficient in adenylate cyclase (EC 4.6.1.1), basal cellular rates of glycogen synthesis were lower and the relative increases produced by exogenous cyclic adenosine 3',5'-monophosphate during growth on glucose were greater than in their respective parent strains. These observations provide strong evidence that endogenous cyclic AMP is one of the key regulators of glycogen synthesis in growing E. coli. In crp mutants, deficient in cyclic AMP receptor protein (CRP), the basal cellular rates of glycogen synthesis were much lower than in their respective parent strains. Stimulation of glycogen synthesis by exogenous cyclic AMP was markedly attenuated in the three crp mutants. Thus, stimulation of glycogen synthesis by either endogenous or exogenous cyclic AMP appears to require CRP. Functional CRP appeared to be required for all three responses observed after cyclic AMP addition: an abrupt step-up in the cellular rate of glycogen synthesis, a continuing exponential increase in rate, and a stimulation of the rate during a subsequent nitrogen starvation. To account for these responses, we derived a mathematical model in which the cyclic AMP-CRP complex regulates the differential rate of synthesis of an enzyme metabolizing an effector of the rate-limiting enzyme of glycogen synthesis. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/6300058/Regulation_of_bacterial_glycogen_synthesis__Stimulation_of_glycogen_synthesis_by_endogenous_and_exogenous_cyclic_adenosine_3':5'_monophosphate_in_Escherichia_coli_and_the_requirement_for_a_functional_CRP_gene_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(18)32739-X DB - PRIME DP - Unbound Medicine ER -