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Effect of acute and chronic cholinesterase inhibition with diisopropylfluorophosphate on muscarinic, dopamine, and GABA receptors of the rat striatum.
J Neurochem. 1983 May; 40(5):1414-22.JN

Abstract

The effects of acute and chronic administration of diisopropylfluorophosphate (DFP) to rats on acetylcholinesterase (AChE) activity (in striatum, medulla, diencephalon, cortex, and medulla) and muscarinic, dopamine (DA), and gamma-aminobutyric acid (GABA) receptor characteristics (in striatum) were investigated. After a single injection of (acute exposure to) DFP, striatal region was found to have the highest degree of AChE inhibition. After daily DFP injections (chronic treatment), all brain regions had the same degree of AChE inhibition, which remained at a steady level despite the regression of the DFP-induced cholinergic overactivity. Acute administration of DFP increased the number of DA and GABA receptors without affecting the muscarinic receptor characteristics. Whereas chronic administration of DFP for either 4 or 14 days reduced the number of muscarinic sites without affecting their affinity, the DFP treatment caused increase in the number of DA and GABA receptors only after 14 days of treatment; however, the increase was considerably lower than that observed after the acute treatment. The in vitro addition of DFP to striatal membranes did not affect DA, GABA, or muscarinic receptors. The results indicate an involvement of GABAergic and dopaminergic systems in the actions of DFP. It is suggested that the GABAergic and dopaminergic involvement may be a part of a compensatory inhibitory process to counteract the excessive cholinergic activity produced by DFP.

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Publisher Full Text

Authors

Sivam SP
No affiliation info available
Norris JC
No affiliation info available
Lim DK
No affiliation info available
Hoskins B
No affiliation info available
Ho IK
No affiliation info available

MeSH

AcetylcholinesteraseAnimalsCorpus StriatumIsoflurophateKineticsMaleMuscimolQuinuclidinyl BenzilateRatsRats, Inbred StrainsReceptors, Cell SurfaceReceptors, CholinergicReceptors, DopamineReceptors, GABA-AReceptors, Muscarinicgamma-Aminobutyric Acid

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

6300336

Citation

Sivam, S P., et al. "Effect of Acute and Chronic Cholinesterase Inhibition With Diisopropylfluorophosphate On Muscarinic, Dopamine, and GABA Receptors of the Rat Striatum." Journal of Neurochemistry, vol. 40, no. 5, 1983, pp. 1414-22.
Sivam SP, Norris JC, Lim DK, et al. Effect of acute and chronic cholinesterase inhibition with diisopropylfluorophosphate on muscarinic, dopamine, and GABA receptors of the rat striatum. J Neurochem. 1983;40(5):1414-22.
Sivam, S. P., Norris, J. C., Lim, D. K., Hoskins, B., & Ho, I. K. (1983). Effect of acute and chronic cholinesterase inhibition with diisopropylfluorophosphate on muscarinic, dopamine, and GABA receptors of the rat striatum. Journal of Neurochemistry, 40(5), 1414-22.
Sivam SP, et al. Effect of Acute and Chronic Cholinesterase Inhibition With Diisopropylfluorophosphate On Muscarinic, Dopamine, and GABA Receptors of the Rat Striatum. J Neurochem. 1983;40(5):1414-22. PubMed PMID: 6300336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of acute and chronic cholinesterase inhibition with diisopropylfluorophosphate on muscarinic, dopamine, and GABA receptors of the rat striatum. AU - Sivam,S P, AU - Norris,J C, AU - Lim,D K, AU - Hoskins,B, AU - Ho,I K, PY - 1983/5/1/pubmed PY - 1983/5/1/medline PY - 1983/5/1/entrez SP - 1414 EP - 22 JF - Journal of neurochemistry JO - J Neurochem VL - 40 IS - 5 N2 - The effects of acute and chronic administration of diisopropylfluorophosphate (DFP) to rats on acetylcholinesterase (AChE) activity (in striatum, medulla, diencephalon, cortex, and medulla) and muscarinic, dopamine (DA), and gamma-aminobutyric acid (GABA) receptor characteristics (in striatum) were investigated. After a single injection of (acute exposure to) DFP, striatal region was found to have the highest degree of AChE inhibition. After daily DFP injections (chronic treatment), all brain regions had the same degree of AChE inhibition, which remained at a steady level despite the regression of the DFP-induced cholinergic overactivity. Acute administration of DFP increased the number of DA and GABA receptors without affecting the muscarinic receptor characteristics. Whereas chronic administration of DFP for either 4 or 14 days reduced the number of muscarinic sites without affecting their affinity, the DFP treatment caused increase in the number of DA and GABA receptors only after 14 days of treatment; however, the increase was considerably lower than that observed after the acute treatment. The in vitro addition of DFP to striatal membranes did not affect DA, GABA, or muscarinic receptors. The results indicate an involvement of GABAergic and dopaminergic systems in the actions of DFP. It is suggested that the GABAergic and dopaminergic involvement may be a part of a compensatory inhibitory process to counteract the excessive cholinergic activity produced by DFP. SN - 0022-3042 UR - https://www.unboundmedicine.com/medline/citation/6300336/Effect_of_acute_and_chronic_cholinesterase_inhibition_with_diisopropylfluorophosphate_on_muscarinic_dopamine_and_GABA_receptors_of_the_rat_striatum_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0022-3042&date=1983&volume=40&issue=5&spage=1414 DB - PRIME DP - Unbound Medicine ER -