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Prostaglandin H2 directly lowers human platelet cAMP levels.
Thromb Res. 1983 Jun 01; 30(5):407-14.TR

Abstract

In the present study we investigated the ability of prostaglandin H2 (PGH2) to lower platelet cAMP levels independent of thromboxane A2 (TXA2) production. Human platelet-rich plasma was incubated (37 degrees C) with 13 nM prostacyclin (PGI2) to increase platelet cAMP levels. Addition of 500 microM arachidonic acid (AA) to PGI2-treated platelets resulted in a return of cAMP to control levels. Inhibition of both PGH2 and TXA2 production with indomethacin (20 microM) blocked the ability of AA to lower PGI2-stimulated increases in cAMP. On the other hand, selective inhibition of TXA2 production with the thromboxane synthetase blocker 7-(1 imidazoyl) heptanoic acid (7-IHA; 10 microM), did not prevent the cAMP lowering effect of added AA. These results provide evidence that PGH2 need not be converted to TXA2 in order to reduce platelet cAMP levels.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6310815

Citation

Rybicki, J P., and G C. Le Breton. "Prostaglandin H2 Directly Lowers Human Platelet cAMP Levels." Thrombosis Research, vol. 30, no. 5, 1983, pp. 407-14.
Rybicki JP, Le Breton GC. Prostaglandin H2 directly lowers human platelet cAMP levels. Thromb Res. 1983;30(5):407-14.
Rybicki, J. P., & Le Breton, G. C. (1983). Prostaglandin H2 directly lowers human platelet cAMP levels. Thrombosis Research, 30(5), 407-14.
Rybicki JP, Le Breton GC. Prostaglandin H2 Directly Lowers Human Platelet cAMP Levels. Thromb Res. 1983 Jun 1;30(5):407-14. PubMed PMID: 6310815.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prostaglandin H2 directly lowers human platelet cAMP levels. AU - Rybicki,J P, AU - Le Breton,G C, PY - 1983/6/1/pubmed PY - 1983/6/1/medline PY - 1983/6/1/entrez SP - 407 EP - 14 JF - Thrombosis research JO - Thromb Res VL - 30 IS - 5 N2 - In the present study we investigated the ability of prostaglandin H2 (PGH2) to lower platelet cAMP levels independent of thromboxane A2 (TXA2) production. Human platelet-rich plasma was incubated (37 degrees C) with 13 nM prostacyclin (PGI2) to increase platelet cAMP levels. Addition of 500 microM arachidonic acid (AA) to PGI2-treated platelets resulted in a return of cAMP to control levels. Inhibition of both PGH2 and TXA2 production with indomethacin (20 microM) blocked the ability of AA to lower PGI2-stimulated increases in cAMP. On the other hand, selective inhibition of TXA2 production with the thromboxane synthetase blocker 7-(1 imidazoyl) heptanoic acid (7-IHA; 10 microM), did not prevent the cAMP lowering effect of added AA. These results provide evidence that PGH2 need not be converted to TXA2 in order to reduce platelet cAMP levels. SN - 0049-3848 UR - https://www.unboundmedicine.com/medline/citation/6310815/Prostaglandin_H2_directly_lowers_human_platelet_cAMP_levels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0049-3848(83)90174-3 DB - PRIME DP - Unbound Medicine ER -