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Binding of intrinsic factor to ileal brush border membrane in the rat.
Biochem Biophys Res Commun. 1983 Aug 30; 115(1):238-44.BB

Abstract

The rat ileal brush border membrane binds both free [125I]-intrinsic factor (IF) and the IF-[57Co]cobalamin (cbl) complex. This binding is observed with IF isolated from rat stomach, but not from IF isolated from hog, canine and human stomachs. The binding of rat-IF[57Co]cbl can be blocked with free rat IF but not with hog IF. The IF-cbl complex binds at a higher affinity (Ka = 0.15 X 10(9) M-1) compared to that of free IF (Ka = 0.9 X 10(9) M-1). Rat IF-cbl also binds efficiently to human and canine ileal membranes. While antibody to the canine ileal receptor blocks the binding of rat, human or hog IF-[57Co]cbl to human and canine ileal membranes, it does not affect the binding of rat IF-[57Co]cbl to rat ileal membranes. These findings demonstrate that the rat ileal receptor is different from canine and human ileal receptors.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6311203

Citation

Seetharam, B, et al. "Binding of Intrinsic Factor to Ileal Brush Border Membrane in the Rat." Biochemical and Biophysical Research Communications, vol. 115, no. 1, 1983, pp. 238-44.
Seetharam B, Bakke JE, Alpers DH. Binding of intrinsic factor to ileal brush border membrane in the rat. Biochem Biophys Res Commun. 1983;115(1):238-44.
Seetharam, B., Bakke, J. E., & Alpers, D. H. (1983). Binding of intrinsic factor to ileal brush border membrane in the rat. Biochemical and Biophysical Research Communications, 115(1), 238-44.
Seetharam B, Bakke JE, Alpers DH. Binding of Intrinsic Factor to Ileal Brush Border Membrane in the Rat. Biochem Biophys Res Commun. 1983 Aug 30;115(1):238-44. PubMed PMID: 6311203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Binding of intrinsic factor to ileal brush border membrane in the rat. AU - Seetharam,B, AU - Bakke,J E, AU - Alpers,D H, PY - 1983/8/30/pubmed PY - 1983/8/30/medline PY - 1983/8/30/entrez SP - 238 EP - 44 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 115 IS - 1 N2 - The rat ileal brush border membrane binds both free [125I]-intrinsic factor (IF) and the IF-[57Co]cobalamin (cbl) complex. This binding is observed with IF isolated from rat stomach, but not from IF isolated from hog, canine and human stomachs. The binding of rat-IF[57Co]cbl can be blocked with free rat IF but not with hog IF. The IF-cbl complex binds at a higher affinity (Ka = 0.15 X 10(9) M-1) compared to that of free IF (Ka = 0.9 X 10(9) M-1). Rat IF-cbl also binds efficiently to human and canine ileal membranes. While antibody to the canine ileal receptor blocks the binding of rat, human or hog IF-[57Co]cbl to human and canine ileal membranes, it does not affect the binding of rat IF-[57Co]cbl to rat ileal membranes. These findings demonstrate that the rat ileal receptor is different from canine and human ileal receptors. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/6311203/Binding_of_intrinsic_factor_to_ileal_brush_border_membrane_in_the_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0006-291X(83)90995-6 DB - PRIME DP - Unbound Medicine ER -