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Enzymic differentiation in cultured foetal hepatocytes of the rat. Induction of serine dehydratase activity by dexamethasone and dibutyryl cyclic AMP.
Differentiation. 1983; 24(3):234-8.D

Abstract

Serine dehydratase activity is absent from the rat foetal liver and normally appears in the immediate postnatal period. In foetal hepatocytes cultured from livers of various gestational ages, enzyme activity can be induced only in the simultaneous presence of dexamethasone and dibutyryl cyclic AMP in the culture medium. Adrenalin and glucagon can replace dibutyryl cyclic AMP. Actinomycin D and cordycepin both repress the response, a result that suggests the induction of enzyme synthesis involves the initial transcription of the enzyme gene(s). Inducibility is assessed in cultures prepared from foetuses aged between 15 and 19 days of gestation after 48 h of culture. No induction is obtained in cells from 15 day foetuses, only a marginal induction from 16 day foetuses, and a substantial induction from older foetuses. In cultures from older foetuses, 6-18 fold inductions are already demonstrable after 24 h of culture. While hepatocytes from more mature foetuses are able to acquire inductibility during culture, cells taken from 15 day foetuses do not develop in the same manner in spite of being maintained under identical conditions. These results suggest that a differentiation event occurs in vivo at about day 16 of foetal development which renders the hepatocyte inducible when cultured. Cells taken prior to this stage do not appear to acquire inducibility. This system represents a case of enzymic differentiation and requires the simultaneous presence of two inducer molecules. The mechanism of induction may represent a unique system in cellular differentiation.

Links

Publisher Full Text
linkinghub.elsevier.com
onlinelibrary.wiley.com

Authors

Oliver IT
No affiliation info available
Martin RL
No affiliation info available
Fisher CJ
No affiliation info available
Yeoh GC
No affiliation info available

MeSH

AnimalsBucladesineCell DifferentiationCells, CulturedDactinomycinDeoxyadenosinesDexamethasoneEnzyme InductionGestational AgeL-Serine DehydrataseLiverRats

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6313460

Citation

Oliver, I T., et al. "Enzymic Differentiation in Cultured Foetal Hepatocytes of the Rat. Induction of Serine Dehydratase Activity By Dexamethasone and Dibutyryl Cyclic AMP." Differentiation; Research in Biological Diversity, vol. 24, no. 3, 1983, pp. 234-8.
Oliver IT, Martin RL, Fisher CJ, et al. Enzymic differentiation in cultured foetal hepatocytes of the rat. Induction of serine dehydratase activity by dexamethasone and dibutyryl cyclic AMP. Differentiation. 1983;24(3):234-8.
Oliver, I. T., Martin, R. L., Fisher, C. J., & Yeoh, G. C. (1983). Enzymic differentiation in cultured foetal hepatocytes of the rat. Induction of serine dehydratase activity by dexamethasone and dibutyryl cyclic AMP. Differentiation; Research in Biological Diversity, 24(3), 234-8.
Oliver IT, et al. Enzymic Differentiation in Cultured Foetal Hepatocytes of the Rat. Induction of Serine Dehydratase Activity By Dexamethasone and Dibutyryl Cyclic AMP. Differentiation. 1983;24(3):234-8. PubMed PMID: 6313460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzymic differentiation in cultured foetal hepatocytes of the rat. Induction of serine dehydratase activity by dexamethasone and dibutyryl cyclic AMP. AU - Oliver,I T, AU - Martin,R L, AU - Fisher,C J, AU - Yeoh,G C, PY - 1983/1/1/pubmed PY - 1983/1/1/medline PY - 1983/1/1/entrez SP - 234 EP - 8 JF - Differentiation; research in biological diversity JO - Differentiation VL - 24 IS - 3 N2 - Serine dehydratase activity is absent from the rat foetal liver and normally appears in the immediate postnatal period. In foetal hepatocytes cultured from livers of various gestational ages, enzyme activity can be induced only in the simultaneous presence of dexamethasone and dibutyryl cyclic AMP in the culture medium. Adrenalin and glucagon can replace dibutyryl cyclic AMP. Actinomycin D and cordycepin both repress the response, a result that suggests the induction of enzyme synthesis involves the initial transcription of the enzyme gene(s). Inducibility is assessed in cultures prepared from foetuses aged between 15 and 19 days of gestation after 48 h of culture. No induction is obtained in cells from 15 day foetuses, only a marginal induction from 16 day foetuses, and a substantial induction from older foetuses. In cultures from older foetuses, 6-18 fold inductions are already demonstrable after 24 h of culture. While hepatocytes from more mature foetuses are able to acquire inductibility during culture, cells taken from 15 day foetuses do not develop in the same manner in spite of being maintained under identical conditions. These results suggest that a differentiation event occurs in vivo at about day 16 of foetal development which renders the hepatocyte inducible when cultured. Cells taken prior to this stage do not appear to acquire inducibility. This system represents a case of enzymic differentiation and requires the simultaneous presence of two inducer molecules. The mechanism of induction may represent a unique system in cellular differentiation. SN - 0301-4681 UR - https://www.unboundmedicine.com/medline/citation/6313460/Enzymic_differentiation_in_cultured_foetal_hepatocytes_of_the_rat__Induction_of_serine_dehydratase_activity_by_dexamethasone_and_dibutyryl_cyclic_AMP_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0301-4681(11)61576-4 DB - PRIME DP - Unbound Medicine ER -