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Arachidonic acid-induced platelet aggregation is mediated by a thromboxane A2/prostaglandin H2 receptor interaction.
J Pharmacol Exp Ther. 1984 Jan; 228(1):240-4.JP

Abstract

The mechanism by which the active metabolites of arachidonic acid (AA), i.e., thromboxane A2 and/or prostaglandin H2 (TXA2/PGH2) induce platelet aggregation is not understood. Several reports have suggested that AA-stimulated aggregation is mediated by secreted ADP, whereas other studies have proposed that this response is ADP-independent. In the present report, we used the specific TXA2/PGH2 receptor antagonist, 13-azaprostanoic acid (13-APA), and the ADP antagonist, ATP, to examine the contribution of TXA2/PGH2 or secreted ADP to aggregation. We found that 13-APA, but not ATP, deaggregates platelets stimulated by AA or U46619 (a TXA2/PGH2 mimetic). In contrast, ADP-induced aggregation was reversed in response to ATP but not to 13-APA. These results suggest that TXA2/PGH2-stimulated aggregation is mediated through TXA2/PGH2 receptor occupation. Furthermore, secreted ADP does not appear to be required for maintenance of the AA-aggregation response.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6319669

Citation

Parise, L V., et al. "Arachidonic Acid-induced Platelet Aggregation Is Mediated By a Thromboxane A2/prostaglandin H2 Receptor Interaction." The Journal of Pharmacology and Experimental Therapeutics, vol. 228, no. 1, 1984, pp. 240-4.
Parise LV, Venton DL, Le Breton GC. Arachidonic acid-induced platelet aggregation is mediated by a thromboxane A2/prostaglandin H2 receptor interaction. J Pharmacol Exp Ther. 1984;228(1):240-4.
Parise, L. V., Venton, D. L., & Le Breton, G. C. (1984). Arachidonic acid-induced platelet aggregation is mediated by a thromboxane A2/prostaglandin H2 receptor interaction. The Journal of Pharmacology and Experimental Therapeutics, 228(1), 240-4.
Parise LV, Venton DL, Le Breton GC. Arachidonic Acid-induced Platelet Aggregation Is Mediated By a Thromboxane A2/prostaglandin H2 Receptor Interaction. J Pharmacol Exp Ther. 1984;228(1):240-4. PubMed PMID: 6319669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Arachidonic acid-induced platelet aggregation is mediated by a thromboxane A2/prostaglandin H2 receptor interaction. AU - Parise,L V, AU - Venton,D L, AU - Le Breton,G C, PY - 1984/1/1/pubmed PY - 1984/1/1/medline PY - 1984/1/1/entrez SP - 240 EP - 4 JF - The Journal of pharmacology and experimental therapeutics JO - J Pharmacol Exp Ther VL - 228 IS - 1 N2 - The mechanism by which the active metabolites of arachidonic acid (AA), i.e., thromboxane A2 and/or prostaglandin H2 (TXA2/PGH2) induce platelet aggregation is not understood. Several reports have suggested that AA-stimulated aggregation is mediated by secreted ADP, whereas other studies have proposed that this response is ADP-independent. In the present report, we used the specific TXA2/PGH2 receptor antagonist, 13-azaprostanoic acid (13-APA), and the ADP antagonist, ATP, to examine the contribution of TXA2/PGH2 or secreted ADP to aggregation. We found that 13-APA, but not ATP, deaggregates platelets stimulated by AA or U46619 (a TXA2/PGH2 mimetic). In contrast, ADP-induced aggregation was reversed in response to ATP but not to 13-APA. These results suggest that TXA2/PGH2-stimulated aggregation is mediated through TXA2/PGH2 receptor occupation. Furthermore, secreted ADP does not appear to be required for maintenance of the AA-aggregation response. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/6319669/Arachidonic_acid_induced_platelet_aggregation_is_mediated_by_a_thromboxane_A2/prostaglandin_H2_receptor_interaction_ L2 - https://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=6319669 DB - PRIME DP - Unbound Medicine ER -