Tags

Type your tag names separated by a space and hit enter

Transferase-deficiency galactosemia: immunochemical studies of the Duarte and Los Angeles variants.
Hum Genet. 1984; 65(3):287-90.HG

Abstract

Rabbit antibodies to purified human placental galactose-1-phosphate uridyltransferase (EC 2.7.7.12) were used to establish immunologic cross-reactivity patterns for the enzyme in hemolysates, prepared from red cells of a normal individual, a homozygous Duarte variant, and a heterozygous Los Angeles variant. The antibody immunoprecipitated all three forms of the enzyme. The amount of antibody absorbed by each hemolysate was related to the different levels of activity, and examination of hemolysate/antibody reaction mixtures by starch gel electrophoresis revealed that the antibody quantitatively precipitated all of the isoenzyme forms that characterize these three genetic variants.

Authors

Andersen MW
No affiliation info available
Williams VP
No affiliation info available
Sparkes MC
No affiliation info available
Sparkes RS
No affiliation info available

MeSH

AnimalsCells, CulturedCross ReactionsElectrophoresis, Starch GelErythrocytesFibroblastsGalactosemiasHeterozygoteHomozygoteHumansIsoenzymesNucleotidyltransferasesPrecipitin TestsRabbitsUTP-Hexose-1-Phosphate Uridylyltransferase

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6321325

Citation

Andersen, M W., et al. "Transferase-deficiency Galactosemia: Immunochemical Studies of the Duarte and Los Angeles Variants." Human Genetics, vol. 65, no. 3, 1984, pp. 287-90.
Andersen MW, Williams VP, Sparkes MC, et al. Transferase-deficiency galactosemia: immunochemical studies of the Duarte and Los Angeles variants. Hum Genet. 1984;65(3):287-90.
Andersen, M. W., Williams, V. P., Sparkes, M. C., & Sparkes, R. S. (1984). Transferase-deficiency galactosemia: immunochemical studies of the Duarte and Los Angeles variants. Human Genetics, 65(3), 287-90.
Andersen MW, et al. Transferase-deficiency Galactosemia: Immunochemical Studies of the Duarte and Los Angeles Variants. Hum Genet. 1984;65(3):287-90. PubMed PMID: 6321325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transferase-deficiency galactosemia: immunochemical studies of the Duarte and Los Angeles variants. AU - Andersen,M W, AU - Williams,V P, AU - Sparkes,M C, AU - Sparkes,R S, PY - 1984/1/1/pubmed PY - 1984/1/1/medline PY - 1984/1/1/entrez SP - 287 EP - 90 JF - Human genetics JO - Hum Genet VL - 65 IS - 3 N2 - Rabbit antibodies to purified human placental galactose-1-phosphate uridyltransferase (EC 2.7.7.12) were used to establish immunologic cross-reactivity patterns for the enzyme in hemolysates, prepared from red cells of a normal individual, a homozygous Duarte variant, and a heterozygous Los Angeles variant. The antibody immunoprecipitated all three forms of the enzyme. The amount of antibody absorbed by each hemolysate was related to the different levels of activity, and examination of hemolysate/antibody reaction mixtures by starch gel electrophoresis revealed that the antibody quantitatively precipitated all of the isoenzyme forms that characterize these three genetic variants. SN - 0340-6717 UR - https://www.unboundmedicine.com/medline/citation/6321325/Transferase_deficiency_galactosemia:_immunochemical_studies_of_the_Duarte_and_Los_Angeles_variants_ L2 - http://www.diseaseinfosearch.org/result/2973 DB - PRIME DP - Unbound Medicine ER -