Tags

Type your tag names separated by a space and hit enter

A study of the effect of alpha-linolenic acid on the desaturation of dihomo-gamma-linolenic acid using rat liver homogenates.
Reprod Nutr Dev. 1984; 24(1):1-10.RN

Abstract

The effects of alpha-linolenic acid on delta 5 desaturation of 20: 3 n-6 dihomo-gamma-linolenic acid) to 20: 4 n-6 (arachidonic acid) and on delta 6 desaturation of 18: 2 n-6 (linoleic acid) to 18:3 n-6 (gamma-linolenic acid) were investigated in two different studies using rat liver homogenates. In the first study, rats were fed a semi-synthetic fat-free diet. When unlabelled 18:3 n-3 was added to the incubation of 14C 18:2 or 14C 20:3, the desaturation rates decreased. delta 6 Desaturation was more strongly inhibited than delta 5 desaturation. The position isomer, gamma-linolenic acid, had no significant effect on delta 6 and delta 5 desaturation. In the second study, the rats were fed a diet including alpha-linolenic acid in the form of rapeseed oil containing no erucic acid. The control animals were fed a peanut oil diet. When alpha-linolenic-fed rats were compared to control animals, we found that delta 6 desaturase activity was lower in the alpha-linolenic-fed animals. In contrast, there was no significant effect on the desaturation rate when 14C 20:3 n-6 was used as a substrate. Our results lead to the conclusion that alpha-linolenic acid, when given to rats as dietary rapeseed oil, partly inhibited delta 6 desaturation in vitro, but did not affect significantly subsequent delta 5 desaturation in the biosynthesis of arachidonic acid. The inhibition of the delta 5 desaturation step, when alpha-linolenic acid was added to the incubation, might be due to an unknown competitive mechanism. These observations have been discussed in relation with the current literature.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6324307

Citation

Blond, J P., and P Lemarchal. "A Study of the Effect of Alpha-linolenic Acid On the Desaturation of Dihomo-gamma-linolenic Acid Using Rat Liver Homogenates." Reproduction, Nutrition, Developpement, vol. 24, no. 1, 1984, pp. 1-10.
Blond JP, Lemarchal P. A study of the effect of alpha-linolenic acid on the desaturation of dihomo-gamma-linolenic acid using rat liver homogenates. Reprod Nutr Dev. 1984;24(1):1-10.
Blond, J. P., & Lemarchal, P. (1984). A study of the effect of alpha-linolenic acid on the desaturation of dihomo-gamma-linolenic acid using rat liver homogenates. Reproduction, Nutrition, Developpement, 24(1), 1-10.
Blond JP, Lemarchal P. A Study of the Effect of Alpha-linolenic Acid On the Desaturation of Dihomo-gamma-linolenic Acid Using Rat Liver Homogenates. Reprod Nutr Dev. 1984;24(1):1-10. PubMed PMID: 6324307.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A study of the effect of alpha-linolenic acid on the desaturation of dihomo-gamma-linolenic acid using rat liver homogenates. AU - Blond,J P, AU - Lemarchal,P, PY - 1984/1/1/pubmed PY - 1984/1/1/medline PY - 1984/1/1/entrez SP - 1 EP - 10 JF - Reproduction, nutrition, developpement JO - Reprod Nutr Dev VL - 24 IS - 1 N2 - The effects of alpha-linolenic acid on delta 5 desaturation of 20: 3 n-6 dihomo-gamma-linolenic acid) to 20: 4 n-6 (arachidonic acid) and on delta 6 desaturation of 18: 2 n-6 (linoleic acid) to 18:3 n-6 (gamma-linolenic acid) were investigated in two different studies using rat liver homogenates. In the first study, rats were fed a semi-synthetic fat-free diet. When unlabelled 18:3 n-3 was added to the incubation of 14C 18:2 or 14C 20:3, the desaturation rates decreased. delta 6 Desaturation was more strongly inhibited than delta 5 desaturation. The position isomer, gamma-linolenic acid, had no significant effect on delta 6 and delta 5 desaturation. In the second study, the rats were fed a diet including alpha-linolenic acid in the form of rapeseed oil containing no erucic acid. The control animals were fed a peanut oil diet. When alpha-linolenic-fed rats were compared to control animals, we found that delta 6 desaturase activity was lower in the alpha-linolenic-fed animals. In contrast, there was no significant effect on the desaturation rate when 14C 20:3 n-6 was used as a substrate. Our results lead to the conclusion that alpha-linolenic acid, when given to rats as dietary rapeseed oil, partly inhibited delta 6 desaturation in vitro, but did not affect significantly subsequent delta 5 desaturation in the biosynthesis of arachidonic acid. The inhibition of the delta 5 desaturation step, when alpha-linolenic acid was added to the incubation, might be due to an unknown competitive mechanism. These observations have been discussed in relation with the current literature. SN - 0181-1916 UR - https://www.unboundmedicine.com/medline/citation/6324307/A_study_of_the_effect_of_alpha_linolenic_acid_on_the_desaturation_of_dihomo_gamma_linolenic_acid_using_rat_liver_homogenates_ L2 - http://publications.edpsciences.org/10.1051/rnd:19840101 DB - PRIME DP - Unbound Medicine ER -