Tags

Type your tag names separated by a space and hit enter

Photodynamic activity of acridine orange: peroxide radical induction in DNA and synthetic polynucleotides.

Abstract

As shown by electron paramagnetic resonance, acridine orange induces the formation of peroxide radicals in DNA when dye-DNA mixtures frozen at 77 K are irradiated with visible light. The reaction is oxygen dependent and strongly reduced by the addition of an electron scavenger. Factors of the medium can modulate the reaction: an ionic strength increased up to 0.3 greatly enhances the dye efficiency whereas the presence of phosphate ions has an inhibiting influence. Acridine orange, which is slightly less efficient than proflavine on native DNA, induces an important peroxide radical formation in poly(dG).poly(dC) but has no action on the poly(dA).poly(dT)polymer.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6327550

Citation

Houba-Hérin, N, et al. "Photodynamic Activity of Acridine Orange: Peroxide Radical Induction in DNA and Synthetic Polynucleotides." International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine, vol. 45, no. 5, 1984, pp. 487-95.
Houba-Hérin N, Calberg-Bacq CM, Van de Vorst A. Photodynamic activity of acridine orange: peroxide radical induction in DNA and synthetic polynucleotides. Int J Radiat Biol Relat Stud Phys Chem Med. 1984;45(5):487-95.
Houba-Hérin, N., Calberg-Bacq, C. M., & Van de Vorst, A. (1984). Photodynamic activity of acridine orange: peroxide radical induction in DNA and synthetic polynucleotides. International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine, 45(5), 487-95.
Houba-Hérin N, Calberg-Bacq CM, Van de Vorst A. Photodynamic Activity of Acridine Orange: Peroxide Radical Induction in DNA and Synthetic Polynucleotides. Int J Radiat Biol Relat Stud Phys Chem Med. 1984;45(5):487-95. PubMed PMID: 6327550.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photodynamic activity of acridine orange: peroxide radical induction in DNA and synthetic polynucleotides. AU - Houba-Hérin,N, AU - Calberg-Bacq,C M, AU - Van de Vorst,A, PY - 1984/5/1/pubmed PY - 1984/5/1/medline PY - 1984/5/1/entrez SP - 487 EP - 95 JF - International journal of radiation biology and related studies in physics, chemistry, and medicine JO - Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. VL - 45 IS - 5 N2 - As shown by electron paramagnetic resonance, acridine orange induces the formation of peroxide radicals in DNA when dye-DNA mixtures frozen at 77 K are irradiated with visible light. The reaction is oxygen dependent and strongly reduced by the addition of an electron scavenger. Factors of the medium can modulate the reaction: an ionic strength increased up to 0.3 greatly enhances the dye efficiency whereas the presence of phosphate ions has an inhibiting influence. Acridine orange, which is slightly less efficient than proflavine on native DNA, induces an important peroxide radical formation in poly(dG).poly(dC) but has no action on the poly(dA).poly(dT)polymer. SN - 0020-7616 UR - https://www.unboundmedicine.com/medline/citation/6327550/Photodynamic_activity_of_acridine_orange:_peroxide_radical_induction_in_DNA_and_synthetic_polynucleotides_ DB - PRIME DP - Unbound Medicine ER -