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Androgen cytosol binding in exercise-induced sparing of muscle atrophy.
Am J Physiol. 1984 Nov; 247(5 Pt 1):E597-603.AJ

Abstract

This study was undertaken to determine whether the exercise-induced sparing of glucocorticoid-induced muscle atrophy is related to increased androgen cytosol binding. Female rats were divided into a sedentary or an exercise group that was trained by treadmill running 100 min/day for 13-15 wk. During the last 12 days of training, each of these groups was further subdivided into groups that received daily subcutaneous injections of cortisone acetate (CA) (100 mg/kg body wt) or the vehicle 1% carboxymethyl cellulose. Exercise prevented 30-40% of the weight loss due to CA treatment in gastrocnemius and plantaris muscles. Scatchard analyses of specific binding of [3H]methyltrienolone (R1881), a synthetic androgen that binds to androgen receptors, were nonlinear in muscles from vehicle-treated sedentary and trained rats and were resolved by a two-component binding model. The lower affinity component, which was attributed to a glucocorticoid receptor, disappeared in muscles of glucocorticoid-treated animals as evidenced by linear Scatchard plots. Receptor concentrations of the androgenic component of [3H]methyltrienolone binding were similar in gastrocnemius and plantaris muscles in all treatment groups. In binding specificity studies of gastrocnemius muscles, the relatively high competition by various glucocorticoids and progesterone for [3H]methyltrienolone binding in the vehicle-treated groups was reduced by CA treatment. The lack of change in androgen cytosol receptor levels suggests that this is not a mechanism by which exercise protects against glucocorticoid-induced muscle atrophy.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6333826

Citation

Hickson, R C., et al. "Androgen Cytosol Binding in Exercise-induced Sparing of Muscle Atrophy." The American Journal of Physiology, vol. 247, no. 5 Pt 1, 1984, pp. E597-603.
Hickson RC, Kurowski TT, Capaccio JA, et al. Androgen cytosol binding in exercise-induced sparing of muscle atrophy. Am J Physiol. 1984;247(5 Pt 1):E597-603.
Hickson, R. C., Kurowski, T. T., Capaccio, J. A., & Chatterton, R. T. (1984). Androgen cytosol binding in exercise-induced sparing of muscle atrophy. The American Journal of Physiology, 247(5 Pt 1), E597-603.
Hickson RC, et al. Androgen Cytosol Binding in Exercise-induced Sparing of Muscle Atrophy. Am J Physiol. 1984;247(5 Pt 1):E597-603. PubMed PMID: 6333826.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Androgen cytosol binding in exercise-induced sparing of muscle atrophy. AU - Hickson,R C, AU - Kurowski,T T, AU - Capaccio,J A, AU - Chatterton,R T,Jr PY - 1984/11/11/pubmed PY - 1984/11/11/medline PY - 1984/11/11/entrez SP - E597 EP - 603 JF - The American journal of physiology JO - Am. J. Physiol. VL - 247 IS - 5 Pt 1 N2 - This study was undertaken to determine whether the exercise-induced sparing of glucocorticoid-induced muscle atrophy is related to increased androgen cytosol binding. Female rats were divided into a sedentary or an exercise group that was trained by treadmill running 100 min/day for 13-15 wk. During the last 12 days of training, each of these groups was further subdivided into groups that received daily subcutaneous injections of cortisone acetate (CA) (100 mg/kg body wt) or the vehicle 1% carboxymethyl cellulose. Exercise prevented 30-40% of the weight loss due to CA treatment in gastrocnemius and plantaris muscles. Scatchard analyses of specific binding of [3H]methyltrienolone (R1881), a synthetic androgen that binds to androgen receptors, were nonlinear in muscles from vehicle-treated sedentary and trained rats and were resolved by a two-component binding model. The lower affinity component, which was attributed to a glucocorticoid receptor, disappeared in muscles of glucocorticoid-treated animals as evidenced by linear Scatchard plots. Receptor concentrations of the androgenic component of [3H]methyltrienolone binding were similar in gastrocnemius and plantaris muscles in all treatment groups. In binding specificity studies of gastrocnemius muscles, the relatively high competition by various glucocorticoids and progesterone for [3H]methyltrienolone binding in the vehicle-treated groups was reduced by CA treatment. The lack of change in androgen cytosol receptor levels suggests that this is not a mechanism by which exercise protects against glucocorticoid-induced muscle atrophy. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/6333826/Androgen_cytosol_binding_in_exercise_induced_sparing_of_muscle_atrophy_ L2 - http://journals.physiology.org/doi/full/10.1152/ajpendo.1984.247.5.E597?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -