Tags

Type your tag names separated by a space and hit enter

NADPH/NADP+ ratio: regulatory implications in yeast glyoxylic acid cycle.
Mol Cell Biochem. 1983; 51(2):123-7.MC

Abstract

The utilization by yeast of two carbon sources is carried out through the operation of the glyoxylic acid cycle. Kinetic acid from the isocitrate transforming enzymes suggest that the flow of isocitrate through the glyoxylic acid cycle depends upon the inhibition of the isocitrate decarboxylating enzymes. Both isocitrate dehydrogenases are inhibited by a mixture of glyoxylate + oxaloacetate, but for the reasons described in the text we consider that this inhibition is of no physiological significance. On the other hand, we have found that NADPH is a competitive inhibitor of NADP-isocitrate dehydrogenase with respect to NADP+, with a KI similar to its KM. It also produces an additive effect on the NADH-produced inhibition of NAD-isocitrate dehydrogenase. We propose NADPH as the compound that channels the utilization of isocitrate into the glyoxylic acid cycle. This is supported by the finding of an increased NADPH/NADP+ ratio in acetate grown yeast with respect to glucose grown cells.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6343836

Citation

Satrustegui, J, et al. "NADPH/NADP+ Ratio: Regulatory Implications in Yeast Glyoxylic Acid Cycle." Molecular and Cellular Biochemistry, vol. 51, no. 2, 1983, pp. 123-7.
Satrustegui J, Bautista J, Machado A. NADPH/NADP+ ratio: regulatory implications in yeast glyoxylic acid cycle. Mol Cell Biochem. 1983;51(2):123-7.
Satrustegui, J., Bautista, J., & Machado, A. (1983). NADPH/NADP+ ratio: regulatory implications in yeast glyoxylic acid cycle. Molecular and Cellular Biochemistry, 51(2), 123-7.
Satrustegui J, Bautista J, Machado A. NADPH/NADP+ Ratio: Regulatory Implications in Yeast Glyoxylic Acid Cycle. Mol Cell Biochem. 1983;51(2):123-7. PubMed PMID: 6343836.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NADPH/NADP+ ratio: regulatory implications in yeast glyoxylic acid cycle. AU - Satrustegui,J, AU - Bautista,J, AU - Machado,A, PY - 1983/1/1/pubmed PY - 1983/1/1/medline PY - 1983/1/1/entrez SP - 123 EP - 7 JF - Molecular and cellular biochemistry JO - Mol Cell Biochem VL - 51 IS - 2 N2 - The utilization by yeast of two carbon sources is carried out through the operation of the glyoxylic acid cycle. Kinetic acid from the isocitrate transforming enzymes suggest that the flow of isocitrate through the glyoxylic acid cycle depends upon the inhibition of the isocitrate decarboxylating enzymes. Both isocitrate dehydrogenases are inhibited by a mixture of glyoxylate + oxaloacetate, but for the reasons described in the text we consider that this inhibition is of no physiological significance. On the other hand, we have found that NADPH is a competitive inhibitor of NADP-isocitrate dehydrogenase with respect to NADP+, with a KI similar to its KM. It also produces an additive effect on the NADH-produced inhibition of NAD-isocitrate dehydrogenase. We propose NADPH as the compound that channels the utilization of isocitrate into the glyoxylic acid cycle. This is supported by the finding of an increased NADPH/NADP+ ratio in acetate grown yeast with respect to glucose grown cells. SN - 0300-8177 UR - https://www.unboundmedicine.com/medline/citation/6343836/NADPH/NADP+_ratio:_regulatory_implications_in_yeast_glyoxylic_acid_cycle_ L2 - https://www.yeastgenome.org/reference/6343836 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.