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Parietal cell surface reactive autoantibody in pernicious anaemia demonstrated by indirect membrane immunofluorescence.
Clin Exp Immunol. 1983 May; 52(2):341-9.CE

Abstract

We examined, in a 'double blind' study, 60 sera from patients with pernicious anaemia for immunofluorescence reactivity with the surface membranes of viable parietal cells isolated from dog stomachs. Fifty-three sera (88%) gave an IgG autoantibody reaction with the surface membranes of parietal cells. Surface staining was also seen with parietal cells from monkey, pig, rat and mouse. The parietal cell surface reactive autoantibody was not found in any of 14 sera from patients with chronic active hepatitis, 10 from patients with systemic lupus erythematosus and 50 from healthy persons. The surface reactivity autoantibody was present in 13 of 14 sera without parietal cell microsomal antibody, 28 of 31 sera without intrinsic factor antibody and in four of four sera without microsomal and intrinsic factor antibodies. Absorption with parietal cell enriched gastric mucosal cells neutralized the activity of the surface reactive but not the microsomal antibody and cross absorption with gastric microsomes neutralized the activity of the microsomal but not the surface reactive antibody. Surface staining of parietal cells was not abolished by absorption with dog or rat hepatocytes, dog or rat kidney cells, human fibroblasts or human AB red blood cells. The results suggest that the parietal cell surface reactive antibody is probably different from the microsomal antibody. Immune reactions of the cell surface reactive antibody with parietal cell surface antigens may play a role in the pathogenesis of the gastric lesion in pernicious anaemia.

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Authors

de Aizpurua HJ
No affiliation info available
Toh BH
No affiliation info available
Ungar B
No affiliation info available

MeSH

AdultAgedAnemia, PerniciousAnimalsAntigens, SurfaceAutoantibodiesCell MembraneDogsFemaleFluorescent Antibody TechniqueGastric MucosaHumansImmunoglobulin GIntrinsic FactorMaleMicrosomesMiddle Aged

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6345039

Citation

de Aizpurua, H J., et al. "Parietal Cell Surface Reactive Autoantibody in Pernicious Anaemia Demonstrated By Indirect Membrane Immunofluorescence." Clinical and Experimental Immunology, vol. 52, no. 2, 1983, pp. 341-9.
de Aizpurua HJ, Toh BH, Ungar B. Parietal cell surface reactive autoantibody in pernicious anaemia demonstrated by indirect membrane immunofluorescence. Clin Exp Immunol. 1983;52(2):341-9.
de Aizpurua, H. J., Toh, B. H., & Ungar, B. (1983). Parietal cell surface reactive autoantibody in pernicious anaemia demonstrated by indirect membrane immunofluorescence. Clinical and Experimental Immunology, 52(2), 341-9.
de Aizpurua HJ, Toh BH, Ungar B. Parietal Cell Surface Reactive Autoantibody in Pernicious Anaemia Demonstrated By Indirect Membrane Immunofluorescence. Clin Exp Immunol. 1983;52(2):341-9. PubMed PMID: 6345039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Parietal cell surface reactive autoantibody in pernicious anaemia demonstrated by indirect membrane immunofluorescence. AU - de Aizpurua,H J, AU - Toh,B H, AU - Ungar,B, PY - 1983/5/1/pubmed PY - 1983/5/1/medline PY - 1983/5/1/entrez SP - 341 EP - 9 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 52 IS - 2 N2 - We examined, in a 'double blind' study, 60 sera from patients with pernicious anaemia for immunofluorescence reactivity with the surface membranes of viable parietal cells isolated from dog stomachs. Fifty-three sera (88%) gave an IgG autoantibody reaction with the surface membranes of parietal cells. Surface staining was also seen with parietal cells from monkey, pig, rat and mouse. The parietal cell surface reactive autoantibody was not found in any of 14 sera from patients with chronic active hepatitis, 10 from patients with systemic lupus erythematosus and 50 from healthy persons. The surface reactivity autoantibody was present in 13 of 14 sera without parietal cell microsomal antibody, 28 of 31 sera without intrinsic factor antibody and in four of four sera without microsomal and intrinsic factor antibodies. Absorption with parietal cell enriched gastric mucosal cells neutralized the activity of the surface reactive but not the microsomal antibody and cross absorption with gastric microsomes neutralized the activity of the microsomal but not the surface reactive antibody. Surface staining of parietal cells was not abolished by absorption with dog or rat hepatocytes, dog or rat kidney cells, human fibroblasts or human AB red blood cells. The results suggest that the parietal cell surface reactive antibody is probably different from the microsomal antibody. Immune reactions of the cell surface reactive antibody with parietal cell surface antigens may play a role in the pathogenesis of the gastric lesion in pernicious anaemia. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/6345039/Parietal_cell_surface_reactive_autoantibody_in_pernicious_anaemia_demonstrated_by_indirect_membrane_immunofluorescence_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/6345039/ DB - PRIME DP - Unbound Medicine ER -