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Efficient production of a ring derivative of chromosome III by the mating-type switching mechanism in Saccharomyces cerevisiae.
Mol Cell Biol. 1983 May; 3(5):803-10.MC

Abstract

The mating-type switches in the yeast Saccharomyces cerevisiae occur by unidirectional transposition of replicas of unexpressed genetic information, residing at HML or HMR, into the mating-type locus (MAT). The source loci, HML and HMR, remain unchanged. Interestingly, when the HM cassettes are expressed, as in marl strains, the HML and HMR cassettes can also efficiently switch, apparently by obtaining genetic information from either of the other two cassettes (Klar et al., Cell 25:517-524, 1981). We have isolated a novel chromosome III rearrangement in heterothallic (marl ho) strains, which is also produced efficiently in marl HO cells, presumably the consequence of a recombination event between HML and HMR. The fusion results in the loss of sequences which are located distal to HML and to HMR and produces a ring derivative of chromosome III. Cells containing such a ring chromosome are viable as haploids; apparently, no essential loci are located distal to the HM loci. The fusion cassette behaves as a standard HM locus with respect to both regulation by the MAR/SIR control and its role in switching MAT.

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Authors

Klar AJ
No affiliation info available
Strathern JN
No affiliation info available
Hicks JB
No affiliation info available
Prudente D
No affiliation info available

MeSH

Base SequenceChromosome AberrationsDNA, FungalGene Expression RegulationGenes, RegulatorRecombination, GeneticReproductionSaccharomyces cerevisiae

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6346056

Citation

Klar, A J., et al. "Efficient Production of a Ring Derivative of Chromosome III By the Mating-type Switching Mechanism in Saccharomyces Cerevisiae." Molecular and Cellular Biology, vol. 3, no. 5, 1983, pp. 803-10.
Klar AJ, Strathern JN, Hicks JB, et al. Efficient production of a ring derivative of chromosome III by the mating-type switching mechanism in Saccharomyces cerevisiae. Mol Cell Biol. 1983;3(5):803-10.
Klar, A. J., Strathern, J. N., Hicks, J. B., & Prudente, D. (1983). Efficient production of a ring derivative of chromosome III by the mating-type switching mechanism in Saccharomyces cerevisiae. Molecular and Cellular Biology, 3(5), 803-10.
Klar AJ, et al. Efficient Production of a Ring Derivative of Chromosome III By the Mating-type Switching Mechanism in Saccharomyces Cerevisiae. Mol Cell Biol. 1983;3(5):803-10. PubMed PMID: 6346056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficient production of a ring derivative of chromosome III by the mating-type switching mechanism in Saccharomyces cerevisiae. AU - Klar,A J, AU - Strathern,J N, AU - Hicks,J B, AU - Prudente,D, PY - 1983/5/1/pubmed PY - 1983/5/1/medline PY - 1983/5/1/entrez SP - 803 EP - 10 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 3 IS - 5 N2 - The mating-type switches in the yeast Saccharomyces cerevisiae occur by unidirectional transposition of replicas of unexpressed genetic information, residing at HML or HMR, into the mating-type locus (MAT). The source loci, HML and HMR, remain unchanged. Interestingly, when the HM cassettes are expressed, as in marl strains, the HML and HMR cassettes can also efficiently switch, apparently by obtaining genetic information from either of the other two cassettes (Klar et al., Cell 25:517-524, 1981). We have isolated a novel chromosome III rearrangement in heterothallic (marl ho) strains, which is also produced efficiently in marl HO cells, presumably the consequence of a recombination event between HML and HMR. The fusion results in the loss of sequences which are located distal to HML and to HMR and produces a ring derivative of chromosome III. Cells containing such a ring chromosome are viable as haploids; apparently, no essential loci are located distal to the HM loci. The fusion cassette behaves as a standard HM locus with respect to both regulation by the MAR/SIR control and its role in switching MAT. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/6346056/Efficient_production_of_a_ring_derivative_of_chromosome_III_by_the_mating_type_switching_mechanism_in_Saccharomyces_cerevisiae_ L2 - https://journals.asm.org/doi/10.1128/mcb.3.5.803-810.1983?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -