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Analysis of wall glucans from yeast, hyphal and germ-tube forming cells of Candida albicans.
J Gen Microbiol. 1984 Dec; 130(12):3295-301.JG

Abstract

Acid-soluble and alkali-insoluble glucan fractions were prepared from yeast, hyphal and germ-tube forming cells of Candida albicans. Alkali-insoluble glucan was also extracted from purified yeast cell walls. Paper chromatography of partial acid hydrolysates confirmed that the glucan preparations contained beta(1----3)- and beta(1----6)-chains but no mixed intra-chain beta(1----3)/(1----6) linkages. Methylation and 13C-NMR analyses showed that the acid-soluble glucan consisted of a highly branched polymer composed mainly (67.0% to 76.6%) of beta(1----6)-linked glucose residues. The alkali-insoluble glucan from yeast and hyphal cells contained from 29.6% to 38.9% beta(1----3) and 43.3% to 53.2% beta(1----6) linkages. Alkali-insoluble glucan from germ-tube forming cells consisted of 67.0% beta(1----3) and 14% beta(1----6) linkages. Branch points accounted for 6.7%, 12.3% and 17.4% of the residues in the alkali-insoluble glucan of yeast, germ-tube forming and hyphal cells, respectively.

Authors

Gopal PK
No affiliation info available
Shepherd MG
No affiliation info available
Sullivan PA
No affiliation info available

MeSH

Candida albicansCell WallChromatography, GasGlucansMagnetic Resonance SpectroscopyMethylationPolysaccharides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6394718

Citation

Gopal, P K., et al. "Analysis of Wall Glucans From Yeast, Hyphal and Germ-tube Forming Cells of Candida Albicans." Journal of General Microbiology, vol. 130, no. 12, 1984, pp. 3295-301.
Gopal PK, Shepherd MG, Sullivan PA. Analysis of wall glucans from yeast, hyphal and germ-tube forming cells of Candida albicans. J Gen Microbiol. 1984;130(12):3295-301.
Gopal, P. K., Shepherd, M. G., & Sullivan, P. A. (1984). Analysis of wall glucans from yeast, hyphal and germ-tube forming cells of Candida albicans. Journal of General Microbiology, 130(12), 3295-301.
Gopal PK, Shepherd MG, Sullivan PA. Analysis of Wall Glucans From Yeast, Hyphal and Germ-tube Forming Cells of Candida Albicans. J Gen Microbiol. 1984;130(12):3295-301. PubMed PMID: 6394718.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of wall glucans from yeast, hyphal and germ-tube forming cells of Candida albicans. AU - Gopal,P K, AU - Shepherd,M G, AU - Sullivan,P A, PY - 1984/12/1/pubmed PY - 1984/12/1/medline PY - 1984/12/1/entrez SP - 3295 EP - 301 JF - Journal of general microbiology JO - J Gen Microbiol VL - 130 IS - 12 N2 - Acid-soluble and alkali-insoluble glucan fractions were prepared from yeast, hyphal and germ-tube forming cells of Candida albicans. Alkali-insoluble glucan was also extracted from purified yeast cell walls. Paper chromatography of partial acid hydrolysates confirmed that the glucan preparations contained beta(1----3)- and beta(1----6)-chains but no mixed intra-chain beta(1----3)/(1----6) linkages. Methylation and 13C-NMR analyses showed that the acid-soluble glucan consisted of a highly branched polymer composed mainly (67.0% to 76.6%) of beta(1----6)-linked glucose residues. The alkali-insoluble glucan from yeast and hyphal cells contained from 29.6% to 38.9% beta(1----3) and 43.3% to 53.2% beta(1----6) linkages. Alkali-insoluble glucan from germ-tube forming cells consisted of 67.0% beta(1----3) and 14% beta(1----6) linkages. Branch points accounted for 6.7%, 12.3% and 17.4% of the residues in the alkali-insoluble glucan of yeast, germ-tube forming and hyphal cells, respectively. SN - 0022-1287 UR - https://www.unboundmedicine.com/medline/citation/6394718/Analysis_of_wall_glucans_from_yeast_hyphal_and_germ_tube_forming_cells_of_Candida_albicans_ DB - PRIME DP - Unbound Medicine ER -