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Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique.
J Clin Microbiol 1983; 17(1):41-7JC

Abstract

A quantitative immunofluorescence assay based on fiber optic microscopy was used to measure the reaction of formalized spores of Bacillus anthracis and Bacillus cereus isolates with fluorescein conjugates prepared by hyperimmunization with B. anthracis Vollum spores. The spores of 11 of the 20 B. cereus strains reacted with the anti-anthrax conjugate to such an extent that they were indistinguishable from the spores of the several B. anthracis isolates tested. However, absorption of the conjugate with spores of B. cereus NCTC 8035 and B. cereus NCTC 10320 greatly reduced the cross-reaction with the B. cereus preparations so that the mean specific fluorescence of samples of B. cereus spores was in no case higher than 14% of the fluorescence of a reference B. anthracis Vollum preparation.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

6402519

Citation

Phillips, A P., et al. "Differentiation Between Spores of Bacillus Anthracis and Bacillus Cereus By a Quantitative Immunofluorescence Technique." Journal of Clinical Microbiology, vol. 17, no. 1, 1983, pp. 41-7.
Phillips AP, Martin KL, Broster MG. Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique. J Clin Microbiol. 1983;17(1):41-7.
Phillips, A. P., Martin, K. L., & Broster, M. G. (1983). Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique. Journal of Clinical Microbiology, 17(1), pp. 41-7.
Phillips AP, Martin KL, Broster MG. Differentiation Between Spores of Bacillus Anthracis and Bacillus Cereus By a Quantitative Immunofluorescence Technique. J Clin Microbiol. 1983;17(1):41-7. PubMed PMID: 6402519.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differentiation between spores of Bacillus anthracis and Bacillus cereus by a quantitative immunofluorescence technique. AU - Phillips,A P, AU - Martin,K L, AU - Broster,M G, PY - 1983/1/1/pubmed PY - 1983/1/1/medline PY - 1983/1/1/entrez SP - 41 EP - 7 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 17 IS - 1 N2 - A quantitative immunofluorescence assay based on fiber optic microscopy was used to measure the reaction of formalized spores of Bacillus anthracis and Bacillus cereus isolates with fluorescein conjugates prepared by hyperimmunization with B. anthracis Vollum spores. The spores of 11 of the 20 B. cereus strains reacted with the anti-anthrax conjugate to such an extent that they were indistinguishable from the spores of the several B. anthracis isolates tested. However, absorption of the conjugate with spores of B. cereus NCTC 8035 and B. cereus NCTC 10320 greatly reduced the cross-reaction with the B. cereus preparations so that the mean specific fluorescence of samples of B. cereus spores was in no case higher than 14% of the fluorescence of a reference B. anthracis Vollum preparation. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/6402519/Differentiation_between_spores_of_Bacillus_anthracis_and_Bacillus_cereus_by_a_quantitative_immunofluorescence_technique_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=6402519 DB - PRIME DP - Unbound Medicine ER -