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Counterimmunoelectrophoresis (immunoelectroosmosis) and serum electrophoretic pattern in serologic diagnosis of canine blastomycosis.
Am J Vet Res. 1983 Feb; 44(2):218-22.AJ

Abstract

Counterimmunoelectrophoresis (CIEP) with blastomyces and histoplasma antigens was used in a serologic study of 181 dogs clinically suspected of having blastomycosis and of 8 dogs with confirmed blastomycosis or histoplasmosis. Thirteen of the 181 dogs, positive by CIEP, were euthanatized, and the diagnosis was confirmed by cultivation and/or microscopic detection of Blastomyces dermatitidis. Additional CIEP-positive dogs were confirmed by staining of aspirates collected in vivo. Radiographic support for the diagnosis was reported in 4 other dogs in which histoplasmosis was excluded by a negative CIEP with histoplasma antigen. The precipitating antibody may disappear during the course of the disease, as it did in 1 dog treated with amphotericin B, but not cured. This dog reverted from CIEP-positive to CIEP-negative within 17 months of treatment (with a weak reaction after 10 months of treatment). The CIEP-detectable antibody was present only in 1 dog without a confirmation by histopathologic findings or cultivation among 24 well-documented cases and 181 total tested sera. The CIEP was more sensitive and specific than was the gel-diffusion precipitin test, eliminated the problems of anticomplementarity that often affected the results of complement-fixation tests with canine sera, and served well in detecting dogs with blastomycosis. Electrophoretic pattern of sera from CIEP-positive dogs with blastomycosis showed a decrease in albumin and an increase in alpha 2- and often in beta- and gamma-globulins, with a substantial decrease of the albumin/globulin ratio.

Authors

Barta O
No affiliation info available
Hubbert NL
No affiliation info available
Pier AC
No affiliation info available
Pourciau SS
No affiliation info available

MeSH

AnimalsBlastomycosisBlood Protein ElectrophoresisComplement Fixation TestsCounterimmunoelectrophoresisDog DiseasesDogsFemaleImmunodiffusionImmunoelectrophoresisMale

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6402960

Citation

Barta, O, et al. "Counterimmunoelectrophoresis (immunoelectroosmosis) and Serum Electrophoretic Pattern in Serologic Diagnosis of Canine Blastomycosis." American Journal of Veterinary Research, vol. 44, no. 2, 1983, pp. 218-22.
Barta O, Hubbert NL, Pier AC, et al. Counterimmunoelectrophoresis (immunoelectroosmosis) and serum electrophoretic pattern in serologic diagnosis of canine blastomycosis. Am J Vet Res. 1983;44(2):218-22.
Barta, O., Hubbert, N. L., Pier, A. C., & Pourciau, S. S. (1983). Counterimmunoelectrophoresis (immunoelectroosmosis) and serum electrophoretic pattern in serologic diagnosis of canine blastomycosis. American Journal of Veterinary Research, 44(2), 218-22.
Barta O, et al. Counterimmunoelectrophoresis (immunoelectroosmosis) and Serum Electrophoretic Pattern in Serologic Diagnosis of Canine Blastomycosis. Am J Vet Res. 1983;44(2):218-22. PubMed PMID: 6402960.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Counterimmunoelectrophoresis (immunoelectroosmosis) and serum electrophoretic pattern in serologic diagnosis of canine blastomycosis. AU - Barta,O, AU - Hubbert,N L, AU - Pier,A C, AU - Pourciau,S S, PY - 1983/2/1/pubmed PY - 1983/2/1/medline PY - 1983/2/1/entrez SP - 218 EP - 22 JF - American journal of veterinary research JO - Am J Vet Res VL - 44 IS - 2 N2 - Counterimmunoelectrophoresis (CIEP) with blastomyces and histoplasma antigens was used in a serologic study of 181 dogs clinically suspected of having blastomycosis and of 8 dogs with confirmed blastomycosis or histoplasmosis. Thirteen of the 181 dogs, positive by CIEP, were euthanatized, and the diagnosis was confirmed by cultivation and/or microscopic detection of Blastomyces dermatitidis. Additional CIEP-positive dogs were confirmed by staining of aspirates collected in vivo. Radiographic support for the diagnosis was reported in 4 other dogs in which histoplasmosis was excluded by a negative CIEP with histoplasma antigen. The precipitating antibody may disappear during the course of the disease, as it did in 1 dog treated with amphotericin B, but not cured. This dog reverted from CIEP-positive to CIEP-negative within 17 months of treatment (with a weak reaction after 10 months of treatment). The CIEP-detectable antibody was present only in 1 dog without a confirmation by histopathologic findings or cultivation among 24 well-documented cases and 181 total tested sera. The CIEP was more sensitive and specific than was the gel-diffusion precipitin test, eliminated the problems of anticomplementarity that often affected the results of complement-fixation tests with canine sera, and served well in detecting dogs with blastomycosis. Electrophoretic pattern of sera from CIEP-positive dogs with blastomycosis showed a decrease in albumin and an increase in alpha 2- and often in beta- and gamma-globulins, with a substantial decrease of the albumin/globulin ratio. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/6402960/Counterimmunoelectrophoresis__immunoelectroosmosis__and_serum_electrophoretic_pattern_in_serologic_diagnosis_of_canine_blastomycosis_ DB - PRIME DP - Unbound Medicine ER -