Tags

Type your tag names separated by a space and hit enter

Serological activity of a characteristic phenolic glycolipid from Mycobacterium leprae in sera from patients with leprosy and tuberculosis.
Clin Exp Immunol. 1983 May; 52(2):271-9.CE

Abstract

Serological activity against a purified phenolic glycolipid from Mycobacterium leprae, which may be obtained in large amounts from M. leprae infected armadillo liver, was investigated using immunodiffusion and an enzyme linked immunosorbent assay (ELISA). Generally a good correlation was obtained between these techniques, but the ELISA was more sensitive and convenient. Relatively high IgG and IgM anti-glycolipid antibody levels were found in lepromatous leprosy patients. The antibody titres to the glycolipid were, however, low when compared with antibody titres to crude sonicates. Since the glycolipid is present in large quantities, this suggests that it is not very immunogenic. Antibody against the glycolipid especially of the IgM class, was demonstrable in some tuberculoid leprosy patients, although at much lower titres than in the lepromatous leprosy sera. In lepromatous leprosy patients that were skin smear negative after more than 5 years of treatment the IgG anti-M. leprae derived glycolipid activity had decreased markedly. The anti-IgG and IgM glycolipid antibody levels in tuberculosis patients did not differ significantly from the levels in appropriate normal healthy subjects. The glycolipid antibody levels in patients infected with M. kansasii, M. avium or M. intracellulare also fell within the range of normal healthy individuals.

Links

PMC Free PDF
PMC Free Full Text

Authors

Brett SJ
No affiliation info available
Draper P
No affiliation info available
Payne SN
No affiliation info available
Rees RJ
No affiliation info available

MeSH

AnimalsAntibodies, BacterialAntigens, BacterialCross ReactionsEnzyme-Linked Immunosorbent AssayGlycolipidsHumansImmunodiffusionImmunoglobulin GImmunoglobulin MLeprosyMiceMycobacterium lepraeRabbitsTuberculosis, Pulmonary

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

6407793

Citation

Brett, S J., et al. "Serological Activity of a Characteristic Phenolic Glycolipid From Mycobacterium Leprae in Sera From Patients With Leprosy and Tuberculosis." Clinical and Experimental Immunology, vol. 52, no. 2, 1983, pp. 271-9.
Brett SJ, Draper P, Payne SN, et al. Serological activity of a characteristic phenolic glycolipid from Mycobacterium leprae in sera from patients with leprosy and tuberculosis. Clin Exp Immunol. 1983;52(2):271-9.
Brett, S. J., Draper, P., Payne, S. N., & Rees, R. J. (1983). Serological activity of a characteristic phenolic glycolipid from Mycobacterium leprae in sera from patients with leprosy and tuberculosis. Clinical and Experimental Immunology, 52(2), 271-9.
Brett SJ, et al. Serological Activity of a Characteristic Phenolic Glycolipid From Mycobacterium Leprae in Sera From Patients With Leprosy and Tuberculosis. Clin Exp Immunol. 1983;52(2):271-9. PubMed PMID: 6407793.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serological activity of a characteristic phenolic glycolipid from Mycobacterium leprae in sera from patients with leprosy and tuberculosis. AU - Brett,S J, AU - Draper,P, AU - Payne,S N, AU - Rees,R J, PY - 1983/5/1/pubmed PY - 1983/5/1/medline PY - 1983/5/1/entrez SP - 271 EP - 9 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 52 IS - 2 N2 - Serological activity against a purified phenolic glycolipid from Mycobacterium leprae, which may be obtained in large amounts from M. leprae infected armadillo liver, was investigated using immunodiffusion and an enzyme linked immunosorbent assay (ELISA). Generally a good correlation was obtained between these techniques, but the ELISA was more sensitive and convenient. Relatively high IgG and IgM anti-glycolipid antibody levels were found in lepromatous leprosy patients. The antibody titres to the glycolipid were, however, low when compared with antibody titres to crude sonicates. Since the glycolipid is present in large quantities, this suggests that it is not very immunogenic. Antibody against the glycolipid especially of the IgM class, was demonstrable in some tuberculoid leprosy patients, although at much lower titres than in the lepromatous leprosy sera. In lepromatous leprosy patients that were skin smear negative after more than 5 years of treatment the IgG anti-M. leprae derived glycolipid activity had decreased markedly. The anti-IgG and IgM glycolipid antibody levels in tuberculosis patients did not differ significantly from the levels in appropriate normal healthy subjects. The glycolipid antibody levels in patients infected with M. kansasii, M. avium or M. intracellulare also fell within the range of normal healthy individuals. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/6407793/Serological_activity_of_a_characteristic_phenolic_glycolipid_from_Mycobacterium_leprae_in_sera_from_patients_with_leprosy_and_tuberculosis_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/6407793/ DB - PRIME DP - Unbound Medicine ER -