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Cloned human interferon-gamma, but not interferon-beta or -alpha, induces expression of HLA-DR determinants by fetal monocytes and myeloid leukemic cell lines.
J Immunol. 1984 Jan; 132(1):240-5.JI

Abstract

The antigen presenting function of macrophages and other accessory cells requires the cell surface expression of class II major histocompatibility antigens, e.g., HLA-DR. It has been shown that gamma-interferon (IFN-gamma), a T cell product, can regulate macrophage HLA-DR expression. The effect of absolutely pure or cloned IFN-gamma upon fetal monocytes that do not strongly express DR has not been studied. We have utilized cloned IFN-gamma, IFN-beta, and IFN-alpha preparations to examine the differential effects of these molecules upon the expression of HLA class I (HLA-A,B) and class II determinants by fetal monocytes as well as by myeloid leukemic cell lines. We report that cloned human IFN-gamma induces the expression of HLA-DR and -A,B antigens on cells that normally express low quantities of these molecules, including human fetal monocytes and two (ML-1 and HL-60) but not a third (U-937) myeloid leukemic cell lines. These findings suggest that the acquisition of class II HLA molecules upon fetal tissues and perhaps antigen presenting function is dependent upon IFN-gamma. In contrast, IFN-beta and -alpha induced the expression of HLA-A,B but not HLA-DR antigens by these cells. Thus, these data indicate that the expression of HLA-DR molecules that is vital for monocyte/macrophage and T cell interaction is stimulated by the lymphokine IFN-gamma, and that the effects of IFN-gamma are distinctive from IFN-alpha or -beta.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6418799

Citation

Kelley, V E., et al. "Cloned Human Interferon-gamma, but Not Interferon-beta or -alpha, Induces Expression of HLA-DR Determinants By Fetal Monocytes and Myeloid Leukemic Cell Lines." Journal of Immunology (Baltimore, Md. : 1950), vol. 132, no. 1, 1984, pp. 240-5.
Kelley VE, Fiers W, Strom TB. Cloned human interferon-gamma, but not interferon-beta or -alpha, induces expression of HLA-DR determinants by fetal monocytes and myeloid leukemic cell lines. J Immunol. 1984;132(1):240-5.
Kelley, V. E., Fiers, W., & Strom, T. B. (1984). Cloned human interferon-gamma, but not interferon-beta or -alpha, induces expression of HLA-DR determinants by fetal monocytes and myeloid leukemic cell lines. Journal of Immunology (Baltimore, Md. : 1950), 132(1), 240-5.
Kelley VE, Fiers W, Strom TB. Cloned Human Interferon-gamma, but Not Interferon-beta or -alpha, Induces Expression of HLA-DR Determinants By Fetal Monocytes and Myeloid Leukemic Cell Lines. J Immunol. 1984;132(1):240-5. PubMed PMID: 6418799.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloned human interferon-gamma, but not interferon-beta or -alpha, induces expression of HLA-DR determinants by fetal monocytes and myeloid leukemic cell lines. AU - Kelley,V E, AU - Fiers,W, AU - Strom,T B, PY - 1984/1/1/pubmed PY - 1984/1/1/medline PY - 1984/1/1/entrez SP - 240 EP - 5 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 132 IS - 1 N2 - The antigen presenting function of macrophages and other accessory cells requires the cell surface expression of class II major histocompatibility antigens, e.g., HLA-DR. It has been shown that gamma-interferon (IFN-gamma), a T cell product, can regulate macrophage HLA-DR expression. The effect of absolutely pure or cloned IFN-gamma upon fetal monocytes that do not strongly express DR has not been studied. We have utilized cloned IFN-gamma, IFN-beta, and IFN-alpha preparations to examine the differential effects of these molecules upon the expression of HLA class I (HLA-A,B) and class II determinants by fetal monocytes as well as by myeloid leukemic cell lines. We report that cloned human IFN-gamma induces the expression of HLA-DR and -A,B antigens on cells that normally express low quantities of these molecules, including human fetal monocytes and two (ML-1 and HL-60) but not a third (U-937) myeloid leukemic cell lines. These findings suggest that the acquisition of class II HLA molecules upon fetal tissues and perhaps antigen presenting function is dependent upon IFN-gamma. In contrast, IFN-beta and -alpha induced the expression of HLA-A,B but not HLA-DR antigens by these cells. Thus, these data indicate that the expression of HLA-DR molecules that is vital for monocyte/macrophage and T cell interaction is stimulated by the lymphokine IFN-gamma, and that the effects of IFN-gamma are distinctive from IFN-alpha or -beta. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/6418799/Cloned_human_interferon_gamma_but_not_interferon_beta_or__alpha_induces_expression_of_HLA_DR_determinants_by_fetal_monocytes_and_myeloid_leukemic_cell_lines_ DB - PRIME DP - Unbound Medicine ER -