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Detection of antibodies to myotoxin a and prairie rattlesnake (Crotalus viridis viridis) venom in three antisera using enzyme-linked immunosorbent assay and immunodiffusion.
Toxicon. 1983; 21(6):849-55.T

Abstract

Immunodiffusion and enzyme-linked immunosorbent assay (ELISA) were used to compare three antisera for their content of antibodies against myotoxin a and C. v. viridis venom. No antibodies were detected in Wyeth's polyvalent (Crotalidae) antivenin against myotoxin a using immunodiffusion, whereas ELISA indicated a low titer of such antibodies. However, antimyotoxin a serum and anti-C. v. viridis venom both had higher titers than antivenin when tested against myotoxin a and crude venom in the ELISA. These results correlate well with previous data which indicated that antiserum to myotoxin a was more effective than antivenin in neutralizing the myotoxicity of C. v. viridis venom. The high content of antibodies to myotoxin a in anti-C. v. viridis venom supports the hypothesis that this monovalent antiserum might be effective in neutralizing rattlesnake (C. v. viridis) venom-induced myonecrosis.

Authors

Ownby CL
No affiliation info available
Odell GV
No affiliation info available
Theakston RD
No affiliation info available

MeSH

AnimalsAntibodiesCrotalid VenomsEnzyme-Linked Immunosorbent AssayImmune SeraImmunodiffusionSnake Venoms

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6419394

Citation

Ownby, C L., et al. "Detection of Antibodies to Myotoxin a and Prairie Rattlesnake (Crotalus Viridis Viridis) Venom in Three Antisera Using Enzyme-linked Immunosorbent Assay and Immunodiffusion." Toxicon : Official Journal of the International Society On Toxinology, vol. 21, no. 6, 1983, pp. 849-55.
Ownby CL, Odell GV, Theakston RD. Detection of antibodies to myotoxin a and prairie rattlesnake (Crotalus viridis viridis) venom in three antisera using enzyme-linked immunosorbent assay and immunodiffusion. Toxicon. 1983;21(6):849-55.
Ownby, C. L., Odell, G. V., & Theakston, R. D. (1983). Detection of antibodies to myotoxin a and prairie rattlesnake (Crotalus viridis viridis) venom in three antisera using enzyme-linked immunosorbent assay and immunodiffusion. Toxicon : Official Journal of the International Society On Toxinology, 21(6), 849-55.
Ownby CL, Odell GV, Theakston RD. Detection of Antibodies to Myotoxin a and Prairie Rattlesnake (Crotalus Viridis Viridis) Venom in Three Antisera Using Enzyme-linked Immunosorbent Assay and Immunodiffusion. Toxicon. 1983;21(6):849-55. PubMed PMID: 6419394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of antibodies to myotoxin a and prairie rattlesnake (Crotalus viridis viridis) venom in three antisera using enzyme-linked immunosorbent assay and immunodiffusion. AU - Ownby,C L, AU - Odell,G V, AU - Theakston,R D, PY - 1983/1/1/pubmed PY - 1983/1/1/medline PY - 1983/1/1/entrez SP - 849 EP - 55 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 21 IS - 6 N2 - Immunodiffusion and enzyme-linked immunosorbent assay (ELISA) were used to compare three antisera for their content of antibodies against myotoxin a and C. v. viridis venom. No antibodies were detected in Wyeth's polyvalent (Crotalidae) antivenin against myotoxin a using immunodiffusion, whereas ELISA indicated a low titer of such antibodies. However, antimyotoxin a serum and anti-C. v. viridis venom both had higher titers than antivenin when tested against myotoxin a and crude venom in the ELISA. These results correlate well with previous data which indicated that antiserum to myotoxin a was more effective than antivenin in neutralizing the myotoxicity of C. v. viridis venom. The high content of antibodies to myotoxin a in anti-C. v. viridis venom supports the hypothesis that this monovalent antiserum might be effective in neutralizing rattlesnake (C. v. viridis) venom-induced myonecrosis. SN - 0041-0101 UR - https://www.unboundmedicine.com/medline/citation/6419394/Detection_of_antibodies_to_myotoxin_a_and_prairie_rattlesnake__Crotalus_viridis_viridis__venom_in_three_antisera_using_enzyme_linked_immunosorbent_assay_and_immunodiffusion_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:6419394 DB - PRIME DP - Unbound Medicine ER -