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Interspecies transduction of plasmids among Bacillus anthracis, B. cereus, and B. thuringiensis.
J Bacteriol. 1984 Mar; 157(3):708-11.JB

Abstract

Bacteriophage CP-51, a generalized transducing phage for Bacillus anthracis, B. cereus, and B. thuringiensis, mediates transduction of plasmid DNA. B. cereus GP7 harbors the 2.8-megadalton multicopy tetracycline resistance plasmid, pBC16. B. thuringiensis 4D11A carries pC194, the 1.8-megadalton multicopy chloramphenicol resistance plasmid. When phage CP-51 was propagated on these strains, it transferred the plasmid-encoded antibiotic resistances to the nonvirulent Weybridge (Sterne) strain of B. anthracis, to B. cereus 569, and to strains of several B. thuringiensis subspecies. The frequency of transfer was as high as 10(-5) transductants per PFU. Tetracycline-resistant and chloramphenicol-resistant transductants contained newly acquired plasmid DNA having the same molecular weight as that contained in the donor strain. Antibiotic-resistant transductants derived from any of the three species were effective donors of plasmids to recipients from all three species.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

6421798

Citation

Ruhfel, R E., et al. "Interspecies Transduction of Plasmids Among Bacillus Anthracis, B. Cereus, and B. Thuringiensis." Journal of Bacteriology, vol. 157, no. 3, 1984, pp. 708-11.
Ruhfel RE, Robillard NJ, Thorne CB. Interspecies transduction of plasmids among Bacillus anthracis, B. cereus, and B. thuringiensis. J Bacteriol. 1984;157(3):708-11.
Ruhfel, R. E., Robillard, N. J., & Thorne, C. B. (1984). Interspecies transduction of plasmids among Bacillus anthracis, B. cereus, and B. thuringiensis. Journal of Bacteriology, 157(3), 708-11.
Ruhfel RE, Robillard NJ, Thorne CB. Interspecies Transduction of Plasmids Among Bacillus Anthracis, B. Cereus, and B. Thuringiensis. J Bacteriol. 1984;157(3):708-11. PubMed PMID: 6421798.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interspecies transduction of plasmids among Bacillus anthracis, B. cereus, and B. thuringiensis. AU - Ruhfel,R E, AU - Robillard,N J, AU - Thorne,C B, PY - 1984/3/1/pubmed PY - 1984/3/1/medline PY - 1984/3/1/entrez SP - 708 EP - 11 JF - Journal of bacteriology JO - J. Bacteriol. VL - 157 IS - 3 N2 - Bacteriophage CP-51, a generalized transducing phage for Bacillus anthracis, B. cereus, and B. thuringiensis, mediates transduction of plasmid DNA. B. cereus GP7 harbors the 2.8-megadalton multicopy tetracycline resistance plasmid, pBC16. B. thuringiensis 4D11A carries pC194, the 1.8-megadalton multicopy chloramphenicol resistance plasmid. When phage CP-51 was propagated on these strains, it transferred the plasmid-encoded antibiotic resistances to the nonvirulent Weybridge (Sterne) strain of B. anthracis, to B. cereus 569, and to strains of several B. thuringiensis subspecies. The frequency of transfer was as high as 10(-5) transductants per PFU. Tetracycline-resistant and chloramphenicol-resistant transductants contained newly acquired plasmid DNA having the same molecular weight as that contained in the donor strain. Antibiotic-resistant transductants derived from any of the three species were effective donors of plasmids to recipients from all three species. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/6421798/Interspecies_transduction_of_plasmids_among_Bacillus_anthracis_B__cereus_and_B__thuringiensis_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=6421798 DB - PRIME DP - Unbound Medicine ER -