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Ia antigen on peripheral blood mononuclear leukocytes in man. II. Functional studies of HLA-DR-positive T cells activated in mixed lymphocyte reactions.
J Exp Med. 1980 Aug 01; 152(2 Pt 2):114s-126s.JE

Abstract

Blast transformation of T cells in response to allogeneic lymphocytes is followed by expression of HLA-DR antigen on up to 60% of the T cells. Murine monoclonal antibody to HLA-DR antigen was used to separate alloactivated T cells into those T cells that express high quantities of DR antigen (DR+) and those that express little or no DR antigen (DR-), and each population was tested in a variety of assays. DR+, but not DR-, T cells stimulated fresh allogeneic and autologous T cells to proliferate and supported proliferation by fresh autologous T cells to soluble antigens. Alloactivated T cells were suppressive of fresh mixed lymphocyte reactions (MLR) and suppression by irradiated DR+ T cells was specific for the DR antigens of the initial stimulator cell. Suppression of the MLR by DR+ T cells was not a result of altered kinetics or cell-mediated cytotoxicity. DR+ T cells released soluble factors that suppressed fresh allogeneic responses. These data indicate that alloactivated DR+ T cells may provide antigen-specific feedback inhibition of the MLR.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6447741

Citation

Engleman, E G., et al. "Ia Antigen On Peripheral Blood Mononuclear Leukocytes in Man. II. Functional Studies of HLA-DR-positive T Cells Activated in Mixed Lymphocyte Reactions." The Journal of Experimental Medicine, vol. 152, no. 2 Pt 2, 1980, 114s-126s.
Engleman EG, Benike CJ, Charron DJ. Ia antigen on peripheral blood mononuclear leukocytes in man. II. Functional studies of HLA-DR-positive T cells activated in mixed lymphocyte reactions. J Exp Med. 1980;152(2 Pt 2):114s-126s.
Engleman, E. G., Benike, C. J., & Charron, D. J. (1980). Ia antigen on peripheral blood mononuclear leukocytes in man. II. Functional studies of HLA-DR-positive T cells activated in mixed lymphocyte reactions. The Journal of Experimental Medicine, 152(2 Pt 2), 114s-126s.
Engleman EG, Benike CJ, Charron DJ. Ia Antigen On Peripheral Blood Mononuclear Leukocytes in Man. II. Functional Studies of HLA-DR-positive T Cells Activated in Mixed Lymphocyte Reactions. J Exp Med. 1980 Aug 1;152(2 Pt 2):114s-126s. PubMed PMID: 6447741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ia antigen on peripheral blood mononuclear leukocytes in man. II. Functional studies of HLA-DR-positive T cells activated in mixed lymphocyte reactions. AU - Engleman,E G, AU - Benike,C J, AU - Charron,D J, PY - 1980/8/1/pubmed PY - 1980/8/1/medline PY - 1980/8/1/entrez SP - 114s EP - 126s JF - The Journal of experimental medicine JO - J Exp Med VL - 152 IS - 2 Pt 2 N2 - Blast transformation of T cells in response to allogeneic lymphocytes is followed by expression of HLA-DR antigen on up to 60% of the T cells. Murine monoclonal antibody to HLA-DR antigen was used to separate alloactivated T cells into those T cells that express high quantities of DR antigen (DR+) and those that express little or no DR antigen (DR-), and each population was tested in a variety of assays. DR+, but not DR-, T cells stimulated fresh allogeneic and autologous T cells to proliferate and supported proliferation by fresh autologous T cells to soluble antigens. Alloactivated T cells were suppressive of fresh mixed lymphocyte reactions (MLR) and suppression by irradiated DR+ T cells was specific for the DR antigens of the initial stimulator cell. Suppression of the MLR by DR+ T cells was not a result of altered kinetics or cell-mediated cytotoxicity. DR+ T cells released soluble factors that suppressed fresh allogeneic responses. These data indicate that alloactivated DR+ T cells may provide antigen-specific feedback inhibition of the MLR. SN - 0022-1007 UR - https://www.unboundmedicine.com/medline/citation/6447741/Ia_antigen_on_peripheral_blood_mononuclear_leukocytes_in_man__II__Functional_studies_of_HLA_DR_positive_T_cells_activated_in_mixed_lymphocyte_reactions_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=6447741.ui DB - PRIME DP - Unbound Medicine ER -