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The dynamics of lipid motion in sarcoplasmic reticulum membranes determined by steady-state and time-resolved fluorescence measurements on 1,6-diphenyl-1,3,5-hexatriene and related molecules.
Biochim Biophys Acta. 1984 Sep 05; 775(3):374-80.BB

Abstract

Steady-state and time-resolved fluorescence anisotropy measurements were made on 1,6-diphenyl-1,3,5-hexatriene (DPH), 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) and 1-acyl-2-(DPH)-phosphatidylcholine (DPH-PC) incorporated into sarcoplasmic reticulum membranes. The results were analysed in terms of the 'wobbling-in-cone' model. Considerable differences in the fluorescence parameters were found. In particular TMA-DPH and DPH-PC showed a smaller cone angle, relating to the range of acyl chain motion, compared to DPH, taken to be a reflection of a difference in probe locations. The influence of the protein component was also found to restrict DPH motion more than TMA-DPH and DPH-PC. Effectiveness in assessment of perturbation of the membrane by the non-esterified fatty acid, oleic acid again revealed differences. The steady-state anisotropy decreased on addition of oleic acid; a recovery to control values was observed with DPH but not with the other probes. Time-resolved parameters followed the same pattern. The results of this work demonstrated the effectiveness of these three probes in revealing differences in membrane properties, such as protein and fatty acid perturbation of membrane lipid structure and dynamics.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6466678

Citation

Stubbs, C D., et al. "The Dynamics of Lipid Motion in Sarcoplasmic Reticulum Membranes Determined By Steady-state and Time-resolved Fluorescence Measurements On 1,6-diphenyl-1,3,5-hexatriene and Related Molecules." Biochimica Et Biophysica Acta, vol. 775, no. 3, 1984, pp. 374-80.
Stubbs CD, Kinosita K, Munkonge F, et al. The dynamics of lipid motion in sarcoplasmic reticulum membranes determined by steady-state and time-resolved fluorescence measurements on 1,6-diphenyl-1,3,5-hexatriene and related molecules. Biochim Biophys Acta. 1984;775(3):374-80.
Stubbs, C. D., Kinosita, K., Munkonge, F., Quinn, P. J., & Ikegami, A. (1984). The dynamics of lipid motion in sarcoplasmic reticulum membranes determined by steady-state and time-resolved fluorescence measurements on 1,6-diphenyl-1,3,5-hexatriene and related molecules. Biochimica Et Biophysica Acta, 775(3), 374-80.
Stubbs CD, et al. The Dynamics of Lipid Motion in Sarcoplasmic Reticulum Membranes Determined By Steady-state and Time-resolved Fluorescence Measurements On 1,6-diphenyl-1,3,5-hexatriene and Related Molecules. Biochim Biophys Acta. 1984 Sep 5;775(3):374-80. PubMed PMID: 6466678.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The dynamics of lipid motion in sarcoplasmic reticulum membranes determined by steady-state and time-resolved fluorescence measurements on 1,6-diphenyl-1,3,5-hexatriene and related molecules. AU - Stubbs,C D, AU - Kinosita,K,Jr AU - Munkonge,F, AU - Quinn,P J, AU - Ikegami,A, PY - 1984/9/5/pubmed PY - 2001/3/28/medline PY - 1984/9/5/entrez SP - 374 EP - 80 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 775 IS - 3 N2 - Steady-state and time-resolved fluorescence anisotropy measurements were made on 1,6-diphenyl-1,3,5-hexatriene (DPH), 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) and 1-acyl-2-(DPH)-phosphatidylcholine (DPH-PC) incorporated into sarcoplasmic reticulum membranes. The results were analysed in terms of the 'wobbling-in-cone' model. Considerable differences in the fluorescence parameters were found. In particular TMA-DPH and DPH-PC showed a smaller cone angle, relating to the range of acyl chain motion, compared to DPH, taken to be a reflection of a difference in probe locations. The influence of the protein component was also found to restrict DPH motion more than TMA-DPH and DPH-PC. Effectiveness in assessment of perturbation of the membrane by the non-esterified fatty acid, oleic acid again revealed differences. The steady-state anisotropy decreased on addition of oleic acid; a recovery to control values was observed with DPH but not with the other probes. Time-resolved parameters followed the same pattern. The results of this work demonstrated the effectiveness of these three probes in revealing differences in membrane properties, such as protein and fatty acid perturbation of membrane lipid structure and dynamics. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/6466678/The_dynamics_of_lipid_motion_in_sarcoplasmic_reticulum_membranes_determined_by_steady_state_and_time_resolved_fluorescence_measurements_on_16_diphenyl_135_hexatriene_and_related_molecules_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0005-2736(84)90193-7 DB - PRIME DP - Unbound Medicine ER -