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The photoreactivity of T-A sequences in oligodeoxyribonucleotides and DNA.
Nucleic Acids Res. 1984 Oct 25; 12(20):7903-14.NA

Abstract

Photoaddition between adjacent adenine and thymine bases occurs, with a quantum yield of approximately 5 X 10(-4) mol einstein-1, when d(T-A), dT-A, d(pT-A), d(T-A-T), d(T-A-T-A) and poly(dA-dT) are irradiated, at 254 nm, in aqueous solution. The photoadduct thus formed is specifically degraded by acid to the fluorescent heterocyclic base 6-methylimidazo[4,5-b]pyridin-5-one (6-MIP) with retention of C(8) of adenine and the methyl group of thymine. This reaction, coupled with either spectrofluorimetric or radiochemical assay of 6-MIP isolated by high voltage paper electrophoresis, has been used to demonstrate formation of the adenine-thymine photoadduct on UV irradiation of poly(dA-dT).poly(dA-dT) and both native and denatured DNA from calf thymus and E. coli. Estimated quantum yields for this new type of photoreaction in DNA show that it is substantially quenched by base pairing. Possible biological implications of the photoreaction are discussed.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6493983

Citation

Bose, S N., and R J. Davies. "The Photoreactivity of T-A Sequences in Oligodeoxyribonucleotides and DNA." Nucleic Acids Research, vol. 12, no. 20, 1984, pp. 7903-14.
Bose SN, Davies RJ. The photoreactivity of T-A sequences in oligodeoxyribonucleotides and DNA. Nucleic Acids Res. 1984;12(20):7903-14.
Bose, S. N., & Davies, R. J. (1984). The photoreactivity of T-A sequences in oligodeoxyribonucleotides and DNA. Nucleic Acids Research, 12(20), 7903-14.
Bose SN, Davies RJ. The Photoreactivity of T-A Sequences in Oligodeoxyribonucleotides and DNA. Nucleic Acids Res. 1984 Oct 25;12(20):7903-14. PubMed PMID: 6493983.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The photoreactivity of T-A sequences in oligodeoxyribonucleotides and DNA. AU - Bose,S N, AU - Davies,R J, PY - 1984/10/25/pubmed PY - 1984/10/25/medline PY - 1984/10/25/entrez SP - 7903 EP - 14 JF - Nucleic acids research JO - Nucleic Acids Res. VL - 12 IS - 20 N2 - Photoaddition between adjacent adenine and thymine bases occurs, with a quantum yield of approximately 5 X 10(-4) mol einstein-1, when d(T-A), dT-A, d(pT-A), d(T-A-T), d(T-A-T-A) and poly(dA-dT) are irradiated, at 254 nm, in aqueous solution. The photoadduct thus formed is specifically degraded by acid to the fluorescent heterocyclic base 6-methylimidazo[4,5-b]pyridin-5-one (6-MIP) with retention of C(8) of adenine and the methyl group of thymine. This reaction, coupled with either spectrofluorimetric or radiochemical assay of 6-MIP isolated by high voltage paper electrophoresis, has been used to demonstrate formation of the adenine-thymine photoadduct on UV irradiation of poly(dA-dT).poly(dA-dT) and both native and denatured DNA from calf thymus and E. coli. Estimated quantum yields for this new type of photoreaction in DNA show that it is substantially quenched by base pairing. Possible biological implications of the photoreaction are discussed. SN - 0305-1048 UR - https://www.unboundmedicine.com/medline/citation/6493983/The_photoreactivity_of_T_A_sequences_in_oligodeoxyribonucleotides_and_DNA_ L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/12.20.7903 DB - PRIME DP - Unbound Medicine ER -