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Separation and analysis of pilocarpine, isopilocarpine, and pilocarpic acid by reverse phase liquid chromatography: collaborative study.
J Assoc Off Anal Chem 1984 Sep-Oct; 67(5):924-6JA

Abstract

A method for separating and determining pilocarpine and 2 degradation products was developed and subjected to collaborative study. Pilocarpine, isopilocarpine, and pilocarpic acid were isolated on a reverse phase liquid chromatographic phenyl bonded column and detected by UV spectrophotometry at 220 nm. Nine collaborators received commercial samples labeled to contain 2, 1, and 0.5% pilocarpine and a 2% practice sample. The collaborative results for pilocarpine were excellent; coefficients of variation ranged from 3.20 to 4.10%. The method was adopted official first action for determination of the active component, pilocarpine, in the presence of isopilocarpine and pilocarpic acid. Although quantitative results for the degradation products were not as good, the method is suitable as a limits test for these substances.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6501156

Citation

Van Ackeren, J L., et al. "Separation and Analysis of Pilocarpine, Isopilocarpine, and Pilocarpic Acid By Reverse Phase Liquid Chromatography: Collaborative Study." Journal - Association of Official Analytical Chemists, vol. 67, no. 5, 1984, pp. 924-6.
Van Ackeren JL, Venable RM, Wainer IW. Separation and analysis of pilocarpine, isopilocarpine, and pilocarpic acid by reverse phase liquid chromatography: collaborative study. J Assoc Off Anal Chem. 1984;67(5):924-6.
Van Ackeren, J. L., Venable, R. M., & Wainer, I. W. (1984). Separation and analysis of pilocarpine, isopilocarpine, and pilocarpic acid by reverse phase liquid chromatography: collaborative study. Journal - Association of Official Analytical Chemists, 67(5), pp. 924-6.
Van Ackeren JL, Venable RM, Wainer IW. Separation and Analysis of Pilocarpine, Isopilocarpine, and Pilocarpic Acid By Reverse Phase Liquid Chromatography: Collaborative Study. J Assoc Off Anal Chem. 1984;67(5):924-6. PubMed PMID: 6501156.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and analysis of pilocarpine, isopilocarpine, and pilocarpic acid by reverse phase liquid chromatography: collaborative study. AU - Van Ackeren,J L, AU - Venable,R M, AU - Wainer,I W, PY - 1984/9/1/pubmed PY - 1984/9/1/medline PY - 1984/9/1/entrez SP - 924 EP - 6 JF - Journal - Association of Official Analytical Chemists JO - J Assoc Off Anal Chem VL - 67 IS - 5 N2 - A method for separating and determining pilocarpine and 2 degradation products was developed and subjected to collaborative study. Pilocarpine, isopilocarpine, and pilocarpic acid were isolated on a reverse phase liquid chromatographic phenyl bonded column and detected by UV spectrophotometry at 220 nm. Nine collaborators received commercial samples labeled to contain 2, 1, and 0.5% pilocarpine and a 2% practice sample. The collaborative results for pilocarpine were excellent; coefficients of variation ranged from 3.20 to 4.10%. The method was adopted official first action for determination of the active component, pilocarpine, in the presence of isopilocarpine and pilocarpic acid. Although quantitative results for the degradation products were not as good, the method is suitable as a limits test for these substances. SN - 0004-5756 UR - https://www.unboundmedicine.com/medline/citation/6501156/Separation_and_analysis_of_pilocarpine_isopilocarpine_and_pilocarpic_acid_by_reverse_phase_liquid_chromatography:_collaborative_study_ DB - PRIME DP - Unbound Medicine ER -