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Discordant expression of human Ia-like antigens on hematopoietic progenitor cells.
J Immunol. 1984 May; 132(5):2324-9.JI

Abstract

The expression of HLA-DR, SB, MT2, and DC antigens on human hematopoietic progenitor cells has been determined by using monoclonal antibodies with complement (C)-mediated cell lysis and immune separation techniques. HLA-DR was detected on greater than 85% of CFU-G/M, myeloid clones (MyCl), BFU-E, and CFU-E. CFU-E were less susceptible to C-mediated lysis at suboptimal C concentrations. The polymorphic MT2 and SB antigens were also present on all categories of progenitor cells, although a lesser proportion of cells were positive. Because in most individuals the antigen density of MT2 and SB, as determined by monoclonal antibody staining, was also lower on B cells and monocytes when compared to HLA-DR expression, the lower number of positive progenitor cells probably reflects lower antigen density rather than distinct positive and negative progenitor cell populations. The DC antigen is expressed weakly on monocytes and B cells, although there is considerable individual variation. In some individuals, distinct DC-positive and -negative monocyte populations are detectable. The DC antigen was not detected on myeloid progenitor cells, even in those individuals with moderate DC expression on their monocytes and B cells. This discordant expression of DC and other Ia-like antigens on hematopoietic progenitor cells may be of physiologic significance and may assist in the purification of progenitor cells from blood and marrow.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6609195

Citation

Linch, D C., et al. "Discordant Expression of Human Ia-like Antigens On Hematopoietic Progenitor Cells." Journal of Immunology (Baltimore, Md. : 1950), vol. 132, no. 5, 1984, pp. 2324-9.
Linch DC, Nadler LM, Luther EA, et al. Discordant expression of human Ia-like antigens on hematopoietic progenitor cells. J Immunol. 1984;132(5):2324-9.
Linch, D. C., Nadler, L. M., Luther, E. A., & Lipton, J. M. (1984). Discordant expression of human Ia-like antigens on hematopoietic progenitor cells. Journal of Immunology (Baltimore, Md. : 1950), 132(5), 2324-9.
Linch DC, et al. Discordant Expression of Human Ia-like Antigens On Hematopoietic Progenitor Cells. J Immunol. 1984;132(5):2324-9. PubMed PMID: 6609195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Discordant expression of human Ia-like antigens on hematopoietic progenitor cells. AU - Linch,D C, AU - Nadler,L M, AU - Luther,E A, AU - Lipton,J M, PY - 1984/5/1/pubmed PY - 1984/5/1/medline PY - 1984/5/1/entrez SP - 2324 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 132 IS - 5 N2 - The expression of HLA-DR, SB, MT2, and DC antigens on human hematopoietic progenitor cells has been determined by using monoclonal antibodies with complement (C)-mediated cell lysis and immune separation techniques. HLA-DR was detected on greater than 85% of CFU-G/M, myeloid clones (MyCl), BFU-E, and CFU-E. CFU-E were less susceptible to C-mediated lysis at suboptimal C concentrations. The polymorphic MT2 and SB antigens were also present on all categories of progenitor cells, although a lesser proportion of cells were positive. Because in most individuals the antigen density of MT2 and SB, as determined by monoclonal antibody staining, was also lower on B cells and monocytes when compared to HLA-DR expression, the lower number of positive progenitor cells probably reflects lower antigen density rather than distinct positive and negative progenitor cell populations. The DC antigen is expressed weakly on monocytes and B cells, although there is considerable individual variation. In some individuals, distinct DC-positive and -negative monocyte populations are detectable. The DC antigen was not detected on myeloid progenitor cells, even in those individuals with moderate DC expression on their monocytes and B cells. This discordant expression of DC and other Ia-like antigens on hematopoietic progenitor cells may be of physiologic significance and may assist in the purification of progenitor cells from blood and marrow. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/6609195/Discordant_expression_of_human_Ia_like_antigens_on_hematopoietic_progenitor_cells_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=6609195 DB - PRIME DP - Unbound Medicine ER -