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Expression of HLA-DR, MB, MT and SB antigens on human mononuclear cells: identification of two phenotypically distinct monocyte populations.
J Immunol. 1984 Sep; 133(3):1300-6.JI

Abstract

The expression of HLA-DR, SB, MB, and MT antigens in different populations of human mononuclear cells was investigated with the use of monoclonal antibodies that recognize distinct human Ia-like antigens. Our results indicate that in man, as previously reported in other species, two phenotypically distinct populations of monocytes or macrophages can be identified on the basis of expression of Class II MHC antigens. Virtually all circulating monocytes displayed determinants associated with HLA-DR, SB, and MT. In addition, a subpopulation of human monocytes expressed MB/DS-associated antigens, as detected with monoclonal antibodies specific for MB1, MB3, and DS-framework determinants. Most B lymphocytes expressed antigens associated with HLA-DR, and the specificities SB2, SB3, MB1, MB3, MT2, and MT3 were also present. Resting T lymphocytes were unreactive with antibodies that recognize all of the Class II MHC antigens tested. T lymphocytes activated by soluble antigen or alloantigens, and expanded in culture, expressed DR, SB, MB, and MT. The majority of the MB/DS+ cells present in the adherent population were monocytes, because they were phagocytic and had the monocyte-specific marker 63D3. The rest of the cells were not identified. They are likely to include mostly B lymphocytes. The presence of other cells, such as dendritic cells, in this subset needs to be determined.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6611370

Citation

Nuñez, G, et al. "Expression of HLA-DR, MB, MT and SB Antigens On Human Mononuclear Cells: Identification of Two Phenotypically Distinct Monocyte Populations." Journal of Immunology (Baltimore, Md. : 1950), vol. 133, no. 3, 1984, pp. 1300-6.
Nuñez G, Giles RC, Ball EJ, et al. Expression of HLA-DR, MB, MT and SB antigens on human mononuclear cells: identification of two phenotypically distinct monocyte populations. J Immunol. 1984;133(3):1300-6.
Nuñez, G., Giles, R. C., Ball, E. J., Hurley, C. K., Capra, J. D., & Stastny, P. (1984). Expression of HLA-DR, MB, MT and SB antigens on human mononuclear cells: identification of two phenotypically distinct monocyte populations. Journal of Immunology (Baltimore, Md. : 1950), 133(3), 1300-6.
Nuñez G, et al. Expression of HLA-DR, MB, MT and SB Antigens On Human Mononuclear Cells: Identification of Two Phenotypically Distinct Monocyte Populations. J Immunol. 1984;133(3):1300-6. PubMed PMID: 6611370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of HLA-DR, MB, MT and SB antigens on human mononuclear cells: identification of two phenotypically distinct monocyte populations. AU - Nuñez,G, AU - Giles,R C, AU - Ball,E J, AU - Hurley,C K, AU - Capra,J D, AU - Stastny,P, PY - 1984/9/1/pubmed PY - 1984/9/1/medline PY - 1984/9/1/entrez SP - 1300 EP - 6 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 133 IS - 3 N2 - The expression of HLA-DR, SB, MB, and MT antigens in different populations of human mononuclear cells was investigated with the use of monoclonal antibodies that recognize distinct human Ia-like antigens. Our results indicate that in man, as previously reported in other species, two phenotypically distinct populations of monocytes or macrophages can be identified on the basis of expression of Class II MHC antigens. Virtually all circulating monocytes displayed determinants associated with HLA-DR, SB, and MT. In addition, a subpopulation of human monocytes expressed MB/DS-associated antigens, as detected with monoclonal antibodies specific for MB1, MB3, and DS-framework determinants. Most B lymphocytes expressed antigens associated with HLA-DR, and the specificities SB2, SB3, MB1, MB3, MT2, and MT3 were also present. Resting T lymphocytes were unreactive with antibodies that recognize all of the Class II MHC antigens tested. T lymphocytes activated by soluble antigen or alloantigens, and expanded in culture, expressed DR, SB, MB, and MT. The majority of the MB/DS+ cells present in the adherent population were monocytes, because they were phagocytic and had the monocyte-specific marker 63D3. The rest of the cells were not identified. They are likely to include mostly B lymphocytes. The presence of other cells, such as dendritic cells, in this subset needs to be determined. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/6611370/Expression_of_HLA_DR_MB_MT_and_SB_antigens_on_human_mononuclear_cells:_identification_of_two_phenotypically_distinct_monocyte_populations_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=6611370 DB - PRIME DP - Unbound Medicine ER -