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Separation of alkenylacyl, alkylacyl, and diacyl analogues and their molecular species by high performance liquid chromatography.
J Lipid Res. 1983 Sep; 24(9):1268-75.JL

Abstract

High performance liquid chromatography methods were established for separation of alkenylacyl, alkylacyl, and diacyl acetylglycerols derived from ethanolamine glycerophospholipids (EGP) and for separation of the individual molecular species from each of the separated classes. The EGP were isolated from bovine brain, hydrolyzed with phospholipase C, and acetylated with acetic anhydride. The three classes of diradylacetylglycerols were separated quantitatively on a mu Porasil silica column. Individual classes were further fractionated on a Zorbax ODS reverse phase column. By gas--liquid chromatographic quantitation of each peak, 29-33 different molecular species were identified within each class. For alkenylacyl-GPE, the major species were 18:1-18:1, 21.8%, and 16:0-18:1, 14.8%. Polyenoic fatty acids predominated at the 2-position of diacyl-GPE. The major species were 18:0-22:6 (n-3), 25.5%, and 18:0-20:4 (n-6), 15.8%. Three species of alkylacyl-GPE, 18:0-20:6 (n-3), 16:0-22:4 (n-6), and 18:0-22:4 (n-6), each accounted for 10%.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6631251

Citation

Nakagawa, Y, and L A. Horrocks. "Separation of Alkenylacyl, Alkylacyl, and Diacyl Analogues and Their Molecular Species By High Performance Liquid Chromatography." Journal of Lipid Research, vol. 24, no. 9, 1983, pp. 1268-75.
Nakagawa Y, Horrocks LA. Separation of alkenylacyl, alkylacyl, and diacyl analogues and their molecular species by high performance liquid chromatography. J Lipid Res. 1983;24(9):1268-75.
Nakagawa, Y., & Horrocks, L. A. (1983). Separation of alkenylacyl, alkylacyl, and diacyl analogues and their molecular species by high performance liquid chromatography. Journal of Lipid Research, 24(9), 1268-75.
Nakagawa Y, Horrocks LA. Separation of Alkenylacyl, Alkylacyl, and Diacyl Analogues and Their Molecular Species By High Performance Liquid Chromatography. J Lipid Res. 1983;24(9):1268-75. PubMed PMID: 6631251.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation of alkenylacyl, alkylacyl, and diacyl analogues and their molecular species by high performance liquid chromatography. AU - Nakagawa,Y, AU - Horrocks,L A, PY - 1983/9/1/pubmed PY - 1983/9/1/medline PY - 1983/9/1/entrez SP - 1268 EP - 75 JF - Journal of lipid research JO - J Lipid Res VL - 24 IS - 9 N2 - High performance liquid chromatography methods were established for separation of alkenylacyl, alkylacyl, and diacyl acetylglycerols derived from ethanolamine glycerophospholipids (EGP) and for separation of the individual molecular species from each of the separated classes. The EGP were isolated from bovine brain, hydrolyzed with phospholipase C, and acetylated with acetic anhydride. The three classes of diradylacetylglycerols were separated quantitatively on a mu Porasil silica column. Individual classes were further fractionated on a Zorbax ODS reverse phase column. By gas--liquid chromatographic quantitation of each peak, 29-33 different molecular species were identified within each class. For alkenylacyl-GPE, the major species were 18:1-18:1, 21.8%, and 16:0-18:1, 14.8%. Polyenoic fatty acids predominated at the 2-position of diacyl-GPE. The major species were 18:0-22:6 (n-3), 25.5%, and 18:0-20:4 (n-6), 15.8%. Three species of alkylacyl-GPE, 18:0-20:6 (n-3), 16:0-22:4 (n-6), and 18:0-22:4 (n-6), each accounted for 10%. SN - 0022-2275 UR - https://www.unboundmedicine.com/medline/citation/6631251/Separation_of_alkenylacyl_alkylacyl_and_diacyl_analogues_and_their_molecular_species_by_high_performance_liquid_chromatography_ L2 - http://www.jlr.org/cgi/pmidlookup?view=long&pmid=6631251 DB - PRIME DP - Unbound Medicine ER -