Effects of alpha-trichosanthin and alpha-momorcharin on the development of peri-implantation mouse embryos.J Reprod Fertil. 1983 Nov; 69(2):597-604.JR
alpha-Trichosanthin (0.3 mg/25 g) and alpha-momorcharin (0.2 mg/25 g) given intraperitoneally to mice on Days 4 and 6 of pregnancy led to an inhibition of implantation. In-vitro study of the effects of these proteins on developing mouse embryos showed that these proteins did not affect the transformation of morulae to blastocysts but further development was impaired: many blastocysts failed to hatch from the zona, the incidence of successful attachment to a plastic substrate decreased and the extent of trophoblastic outgrowth diminished. Inner cell mass development was less affected than was the trophoblast. The in-vivo inhibition of implantation may therefore be a consequence of the deleterious effect of these proteins on the trophoblast.