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[Correlation of biliary cholesterol, phospholipids and bile acid compositions and the development of cholesterol cholelithiasis in mice].
Nihon Yakurigaku Zasshi 1983; 82(3):171-80NY

Abstract

A study was attempted to establish a screening method for detecting cholelitholytic ingredients from a wide variety of natural substances. Although mice were selected as a suitable pathological model of cholelithiasis to detect a small amount of the ingredients, all the conventional lithogenic diets caused unfavorable influence on the animals. Therefore, as the first step we formulated a new lithogenic diet consisting of butter, cholesterol, cholic acid, etc, which was adequate for mice. Subsequently, the pathological characteristics and persistence of cholelithiasis were examined in the animals; the changes in bile compositions including free and conjugated bile acids, cholesterol and phospholipids were observed before and at the onset of cholelithiasis. Following confirmation of the stone formation, a normal diet was substituted for the lithogenic diet to likewise assess the bile compositions 4 and 6 weeks later. An increasing tendency for deoxycholic acid, disappearance of chenodeoxycholic acid and decrease in ursodeoxycholic acid were seen under the condition of cholelithiasis. In addition, the cholic acid-glycine conjugate which should not exist in the normal state and the increase in free and tauring-conjugated cholic acid were noticed. The biliary cholesterol level in treated mice increased to about 4 times higher than that in untreated mice, while the biliary phospholipids and total bile acids levels increased to only about 1.5 and about 2 times the control levels, respectively. The incidence of stone formation rose sharply at an experimental period between 2 and 3 weeks after starting the lithogenic diet. Gallstones die not disappear even at the 6th week after substituting a normal diet for the lithogenic one. However, the cholic acid-glycine conjugate disappeared, and deoxycholic acid as well as chenodeoxycholic acid and ursodeoxycholic acid tended to recover to the normal levels in the bile.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

jpn

PubMed ID

6662416

Citation

Yamahara, J, et al. "[Correlation of Biliary Cholesterol, Phospholipids and Bile Acid Compositions and the Development of Cholesterol Cholelithiasis in Mice]." Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica, vol. 82, no. 3, 1983, pp. 171-80.
Yamahara J, Chisaka T, Sawada T, et al. [Correlation of biliary cholesterol, phospholipids and bile acid compositions and the development of cholesterol cholelithiasis in mice]. Nippon Yakurigaku Zasshi. 1983;82(3):171-80.
Yamahara, J., Chisaka, T., Sawada, T., & Fujimura, H. (1983). [Correlation of biliary cholesterol, phospholipids and bile acid compositions and the development of cholesterol cholelithiasis in mice]. Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica, 82(3), pp. 171-80.
Yamahara J, et al. [Correlation of Biliary Cholesterol, Phospholipids and Bile Acid Compositions and the Development of Cholesterol Cholelithiasis in Mice]. Nippon Yakurigaku Zasshi. 1983;82(3):171-80. PubMed PMID: 6662416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Correlation of biliary cholesterol, phospholipids and bile acid compositions and the development of cholesterol cholelithiasis in mice]. AU - Yamahara,J, AU - Chisaka,T, AU - Sawada,T, AU - Fujimura,H, PY - 1983/9/1/pubmed PY - 1983/9/1/medline PY - 1983/9/1/entrez SP - 171 EP - 80 JF - Nihon yakurigaku zasshi. Folia pharmacologica Japonica JO - Nippon Yakurigaku Zasshi VL - 82 IS - 3 N2 - A study was attempted to establish a screening method for detecting cholelitholytic ingredients from a wide variety of natural substances. Although mice were selected as a suitable pathological model of cholelithiasis to detect a small amount of the ingredients, all the conventional lithogenic diets caused unfavorable influence on the animals. Therefore, as the first step we formulated a new lithogenic diet consisting of butter, cholesterol, cholic acid, etc, which was adequate for mice. Subsequently, the pathological characteristics and persistence of cholelithiasis were examined in the animals; the changes in bile compositions including free and conjugated bile acids, cholesterol and phospholipids were observed before and at the onset of cholelithiasis. Following confirmation of the stone formation, a normal diet was substituted for the lithogenic diet to likewise assess the bile compositions 4 and 6 weeks later. An increasing tendency for deoxycholic acid, disappearance of chenodeoxycholic acid and decrease in ursodeoxycholic acid were seen under the condition of cholelithiasis. In addition, the cholic acid-glycine conjugate which should not exist in the normal state and the increase in free and tauring-conjugated cholic acid were noticed. The biliary cholesterol level in treated mice increased to about 4 times higher than that in untreated mice, while the biliary phospholipids and total bile acids levels increased to only about 1.5 and about 2 times the control levels, respectively. The incidence of stone formation rose sharply at an experimental period between 2 and 3 weeks after starting the lithogenic diet. Gallstones die not disappear even at the 6th week after substituting a normal diet for the lithogenic one. However, the cholic acid-glycine conjugate disappeared, and deoxycholic acid as well as chenodeoxycholic acid and ursodeoxycholic acid tended to recover to the normal levels in the bile. SN - 0015-5691 UR - https://www.unboundmedicine.com/medline/citation/6662416/[Correlation_of_biliary_cholesterol_phospholipids_and_bile_acid_compositions_and_the_development_of_cholesterol_cholelithiasis_in_mice]_ L2 - https://medlineplus.gov/cholesterollevelswhatyouneedtoknow.html DB - PRIME DP - Unbound Medicine ER -