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The stability of tissue plasminogen activator (t-PA).
Thromb Haemost. 1983 Oct 31; 50(3):650-1.TH

Abstract

Since tissue-type plasminogen activator (t-PA) derived from tissue culture and recombinant DNA procedures has been proposed for use in thrombolytic therapy in man, it is essential that the t-PA molecule should display reasonable stability in a lyophilised state to facilitate its usefulness. In this study, four laboratories compared the potencies of three preparations of t-PA following storage at 4 degrees, 20 degrees, 37 degrees and 45 degrees C, using each t-PA stored at -20 degrees C as a reference (100% activity) in each case. A pig heart extract of t-PA was the most stable, losing no activity when stored for 30 days at 37 degrees C, while two melanoma cell tissue culture extracts varied in their storage behaviour. One was quite stable at 37 degrees C (losing about 3% of its activity) while the other lost about 16%. Thus both the pig heart t-PA and one t-PA from melanoma cell culture proved suitable for the further development of reference standards for t-PA activity.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

6685926

Citation

Gaffney, P J., et al. "The Stability of Tissue Plasminogen Activator (t-PA)." Thrombosis and Haemostasis, vol. 50, no. 3, 1983, pp. 650-1.
Gaffney PJ, Templeman J, Curtis AD, et al. The stability of tissue plasminogen activator (t-PA). Thromb Haemost. 1983;50(3):650-1.
Gaffney, P. J., Templeman, J., Curtis, A. D., & Campbell, P. J. (1983). The stability of tissue plasminogen activator (t-PA). Thrombosis and Haemostasis, 50(3), 650-1.
Gaffney PJ, et al. The Stability of Tissue Plasminogen Activator (t-PA). Thromb Haemost. 1983 Oct 31;50(3):650-1. PubMed PMID: 6685926.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The stability of tissue plasminogen activator (t-PA). AU - Gaffney,P J, AU - Templeman,J, AU - Curtis,A D, AU - Campbell,P J, PY - 1983/10/31/pubmed PY - 1983/10/31/medline PY - 1983/10/31/entrez SP - 650 EP - 1 JF - Thrombosis and haemostasis JO - Thromb. Haemost. VL - 50 IS - 3 N2 - Since tissue-type plasminogen activator (t-PA) derived from tissue culture and recombinant DNA procedures has been proposed for use in thrombolytic therapy in man, it is essential that the t-PA molecule should display reasonable stability in a lyophilised state to facilitate its usefulness. In this study, four laboratories compared the potencies of three preparations of t-PA following storage at 4 degrees, 20 degrees, 37 degrees and 45 degrees C, using each t-PA stored at -20 degrees C as a reference (100% activity) in each case. A pig heart extract of t-PA was the most stable, losing no activity when stored for 30 days at 37 degrees C, while two melanoma cell tissue culture extracts varied in their storage behaviour. One was quite stable at 37 degrees C (losing about 3% of its activity) while the other lost about 16%. Thus both the pig heart t-PA and one t-PA from melanoma cell culture proved suitable for the further development of reference standards for t-PA activity. SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/6685926/The_stability_of_tissue_plasminogen_activator__t_PA__ DB - PRIME DP - Unbound Medicine ER -