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Improved liquid-chromatographic determination of mexiletine, an antiarrhythmic drug, in plasma.
Clin Chem. 1984 Feb; 30(2):319-22.CC

Abstract

In this improved reversed-phase liquid-chromatographic procedure for determination of mexiletine in plasma, mexiletine and an internal standard, chlorodisopyramide, are extracted with methylene chloride from 0.5 mL of serum or plasma; the extract is then concentrated and injected onto a C18 chromatographic column. Mexiletine in the column effluent is detected by monitoring absorbance at 210 nm. It is quantified by use of mexiletine-internal standard peak-height ratios. The relation between this ratio and mexiletine concentration is linear from 0.1 to 5.0 mg/L. The lower limit of detection is about 50 micrograms/L. At a mexiletine concentration of 2.0 mg/L in serum, intrarun precision (CV) is 2.9% and inter-run precision is 5.9%; at 0.5 mg/L, these CVs are 5.7% and 9.6%, respectively. Analytical recovery of added mexiletine in serum is 68-88%. Therapeutic concentrations of some commonly administered drugs in patients' specimens did not interfere. In serum from 38 patients receiving mexiletine for cardiac arrhythmia, concentrations measured by this method correlated with therapeutic efficacy.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

6692546

Citation

Mastropaolo, W, et al. "Improved Liquid-chromatographic Determination of Mexiletine, an Antiarrhythmic Drug, in Plasma." Clinical Chemistry, vol. 30, no. 2, 1984, pp. 319-22.
Mastropaolo W, Holmes DR, Osborn MJ, et al. Improved liquid-chromatographic determination of mexiletine, an antiarrhythmic drug, in plasma. Clin Chem. 1984;30(2):319-22.
Mastropaolo, W., Holmes, D. R., Osborn, M. J., Rooke, J., & Moyer, T. P. (1984). Improved liquid-chromatographic determination of mexiletine, an antiarrhythmic drug, in plasma. Clinical Chemistry, 30(2), 319-22.
Mastropaolo W, et al. Improved Liquid-chromatographic Determination of Mexiletine, an Antiarrhythmic Drug, in Plasma. Clin Chem. 1984;30(2):319-22. PubMed PMID: 6692546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved liquid-chromatographic determination of mexiletine, an antiarrhythmic drug, in plasma. AU - Mastropaolo,W, AU - Holmes,D R, AU - Osborn,M J, AU - Rooke,J, AU - Moyer,T P, PY - 1984/2/1/pubmed PY - 1984/2/1/medline PY - 1984/2/1/entrez SP - 319 EP - 22 JF - Clinical chemistry JO - Clin. Chem. VL - 30 IS - 2 N2 - In this improved reversed-phase liquid-chromatographic procedure for determination of mexiletine in plasma, mexiletine and an internal standard, chlorodisopyramide, are extracted with methylene chloride from 0.5 mL of serum or plasma; the extract is then concentrated and injected onto a C18 chromatographic column. Mexiletine in the column effluent is detected by monitoring absorbance at 210 nm. It is quantified by use of mexiletine-internal standard peak-height ratios. The relation between this ratio and mexiletine concentration is linear from 0.1 to 5.0 mg/L. The lower limit of detection is about 50 micrograms/L. At a mexiletine concentration of 2.0 mg/L in serum, intrarun precision (CV) is 2.9% and inter-run precision is 5.9%; at 0.5 mg/L, these CVs are 5.7% and 9.6%, respectively. Analytical recovery of added mexiletine in serum is 68-88%. Therapeutic concentrations of some commonly administered drugs in patients' specimens did not interfere. In serum from 38 patients receiving mexiletine for cardiac arrhythmia, concentrations measured by this method correlated with therapeutic efficacy. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/6692546/Improved_liquid_chromatographic_determination_of_mexiletine_an_antiarrhythmic_drug_in_plasma_ L2 - http://www.clinchem.org/cgi/pmidlookup?view=long&pmid=6692546 DB - PRIME DP - Unbound Medicine ER -