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Multicomponent analysis of hemoglobin derivatives with reversed-optics spectrophotometer.
Clin Chem. 1984 Mar; 30(3):373-9.CC

Abstract

We describe a method for the simultaneous determination of the five clinically relevant hemoglobin derivatives (Hb, HbO2, HbCO, Hi, SHb) in a blood sample by means of a reversed-optics spectrophotometer (Hewlett-Packard HP8450 A UV/Vis). A built-in computer program is used for multicomponent analysis in an overdetermined system, i.e., a system in which the number of independent equations used exceeds the number of unknowns to be determined. First, the spectra of the five hemoglobin derivatives are measured in a series of different human blood samples. Thereafter, the multicomponent method for the simultaneous determination of the five hemoglobin derivatives is tested by comparison with conventional methods for the separate determination of oxygen saturation, HbCO, Hi, and SHb fractions. The multicomponent (multiwavelength) method is sufficiently reliable, accurate, and easy to justify its use in physiological chemical research as well as its routine application in the clinical chemical laboratory.

Authors

Zwart A
No affiliation info available
Buursma A
No affiliation info available
van Kampen EJ
No affiliation info available
Zijlstra WG
No affiliation info available

MeSH

CarboxyhemoglobinComputersFemaleHemoglobinsHumansMaleMethemoglobinOxyhemoglobinsSpectrophotometrySulfhemoglobin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6697482

Citation

Zwart, A, et al. "Multicomponent Analysis of Hemoglobin Derivatives With Reversed-optics Spectrophotometer." Clinical Chemistry, vol. 30, no. 3, 1984, pp. 373-9.
Zwart A, Buursma A, van Kampen EJ, et al. Multicomponent analysis of hemoglobin derivatives with reversed-optics spectrophotometer. Clin Chem. 1984;30(3):373-9.
Zwart, A., Buursma, A., van Kampen, E. J., & Zijlstra, W. G. (1984). Multicomponent analysis of hemoglobin derivatives with reversed-optics spectrophotometer. Clinical Chemistry, 30(3), 373-9.
Zwart A, et al. Multicomponent Analysis of Hemoglobin Derivatives With Reversed-optics Spectrophotometer. Clin Chem. 1984;30(3):373-9. PubMed PMID: 6697482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multicomponent analysis of hemoglobin derivatives with reversed-optics spectrophotometer. AU - Zwart,A, AU - Buursma,A, AU - van Kampen,E J, AU - Zijlstra,W G, PY - 1984/3/1/pubmed PY - 1984/3/1/medline PY - 1984/3/1/entrez SP - 373 EP - 9 JF - Clinical chemistry JO - Clin Chem VL - 30 IS - 3 N2 - We describe a method for the simultaneous determination of the five clinically relevant hemoglobin derivatives (Hb, HbO2, HbCO, Hi, SHb) in a blood sample by means of a reversed-optics spectrophotometer (Hewlett-Packard HP8450 A UV/Vis). A built-in computer program is used for multicomponent analysis in an overdetermined system, i.e., a system in which the number of independent equations used exceeds the number of unknowns to be determined. First, the spectra of the five hemoglobin derivatives are measured in a series of different human blood samples. Thereafter, the multicomponent method for the simultaneous determination of the five hemoglobin derivatives is tested by comparison with conventional methods for the separate determination of oxygen saturation, HbCO, Hi, and SHb fractions. The multicomponent (multiwavelength) method is sufficiently reliable, accurate, and easy to justify its use in physiological chemical research as well as its routine application in the clinical chemical laboratory. SN - 0009-9147 UR - https://www.unboundmedicine.com/medline/citation/6697482/Multicomponent_analysis_of_hemoglobin_derivatives_with_reversed_optics_spectrophotometer_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:6697482 DB - PRIME DP - Unbound Medicine ER -