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Structure and antitumor activity of an alkali-soluble polysaccharide from Cordyceps ophioglossoides.
Carbohydr Res. 1984 Jan 10; 125(1):107-15.CR

Abstract

A water-insoluble extracellular glucan (CO-1) was isolated from the precipitate formed on incubation of the culture filtrate of Cordyceps ophioglossoides at 37 degrees for 19 h. CO-1 was homogeneous as judged by h.p.l.c., electrophoresis, and ultracentrifugation, and the average molecular weight was determined by h.p.l.c. to be 632,000. The 1H- and 13C-n.m.r. and the i.r. spectra indicated that the glucosidic linkages in CO-1 were beta. From the results of methylation analysis, Smith degradation, n.m.r. studies, and enzymic hydrolysis, it was concluded that CO-1 is composed of a backbone of (1----3)-linked beta-D-glucopyranosyl residues with a beta-D-glucopyranosyl group attached to O-6 of every second D-glucopyranosyl residue of the backbone. CO-1 strongly inhibited the growth of Sarcoma 180 solid-type tumor. CO-1 polyalcohol, which was prepared by Smith degradation of CO-1, exhibited more-potent antitumor activity than CO-1. The absorption maximum of Congo Red shifted significantly in the presence of CO-1.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

6704989

Citation

Yamada, H, et al. "Structure and Antitumor Activity of an Alkali-soluble Polysaccharide From Cordyceps Ophioglossoides." Carbohydrate Research, vol. 125, no. 1, 1984, pp. 107-15.
Yamada H, Kawaguchi N, Ohmori T, et al. Structure and antitumor activity of an alkali-soluble polysaccharide from Cordyceps ophioglossoides. Carbohydr Res. 1984;125(1):107-15.
Yamada, H., Kawaguchi, N., Ohmori, T., Takeshita, Y., Taneya, S., & Miyazaki, T. (1984). Structure and antitumor activity of an alkali-soluble polysaccharide from Cordyceps ophioglossoides. Carbohydrate Research, 125(1), 107-15.
Yamada H, et al. Structure and Antitumor Activity of an Alkali-soluble Polysaccharide From Cordyceps Ophioglossoides. Carbohydr Res. 1984 Jan 10;125(1):107-15. PubMed PMID: 6704989.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structure and antitumor activity of an alkali-soluble polysaccharide from Cordyceps ophioglossoides. AU - Yamada,H, AU - Kawaguchi,N, AU - Ohmori,T, AU - Takeshita,Y, AU - Taneya,S, AU - Miyazaki,T, PY - 1984/1/10/pubmed PY - 1984/1/10/medline PY - 1984/1/10/entrez SP - 107 EP - 15 JF - Carbohydrate research JO - Carbohydr Res VL - 125 IS - 1 N2 - A water-insoluble extracellular glucan (CO-1) was isolated from the precipitate formed on incubation of the culture filtrate of Cordyceps ophioglossoides at 37 degrees for 19 h. CO-1 was homogeneous as judged by h.p.l.c., electrophoresis, and ultracentrifugation, and the average molecular weight was determined by h.p.l.c. to be 632,000. The 1H- and 13C-n.m.r. and the i.r. spectra indicated that the glucosidic linkages in CO-1 were beta. From the results of methylation analysis, Smith degradation, n.m.r. studies, and enzymic hydrolysis, it was concluded that CO-1 is composed of a backbone of (1----3)-linked beta-D-glucopyranosyl residues with a beta-D-glucopyranosyl group attached to O-6 of every second D-glucopyranosyl residue of the backbone. CO-1 strongly inhibited the growth of Sarcoma 180 solid-type tumor. CO-1 polyalcohol, which was prepared by Smith degradation of CO-1, exhibited more-potent antitumor activity than CO-1. The absorption maximum of Congo Red shifted significantly in the presence of CO-1. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/6704989/Structure_and_antitumor_activity_of_an_alkali_soluble_polysaccharide_from_Cordyceps_ophioglossoides_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0008-6215(84)85146-0 DB - PRIME DP - Unbound Medicine ER -