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Orthograde and retrograde axoplasmic transport during acute ocular hypertension in the monkey.
Invest Ophthalmol Vis Sci. 1977 May; 16(5):426-41.IO

Abstract

Orthograde and retrograde axoplasmic transport have been studied in the optic nerve heads of 37 Macaca fascicularis eyes with normal or elevated intraocular pressure (IOP) produced by cannulation of the anterior chamber. Orthograde transport was labeled by 3H-amino acids injected intravitreally and incorporated into retinal ganglion cell proteins. Retrograde transport was studied in the same eyes by injecting horseradish peroxidase (HRP) into one or both optic tracts and dorsal lateral geniculate nuclei (dLGN). Both tracers accumulated in the lamina scleralis (LS) of eyes maintained at pressures of 25 to 150 mm. Hg for 12 to 28 hours (pressure in normal controls = 10 to 14 mm. Hg) but the HRP technique was markedly more sensitive. The degree of retrograde transport obstruction in the LS appeared to be directly proportional to both the height and the duration of elevated IOP. In one experiment, the blockades of orthograde and retrograde transport induced at 50 mm. Hg were demonstrated to be reversible. Serial reconstructions of radioautographs and peroxidase-reacted sections of the optic nerve heads demonstrated that the orthograde and retrograde transport obstructions were coincidental anatomically by light microscopy in the LS and occurred most prominently in the temporal quadrants of the nerve head. These transport obstructions occurred at moderate elevations of IOP (25 TO 50 mm. Hg) despite (1) elevated arterial PO2 levels during inhalation of 100 percent oxygen and (2) intact nerve head capillary circulation, as demonstrated by perfusion with nucleated avian erythrocytes.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

67096

Citation

Minckler, D S., et al. "Orthograde and Retrograde Axoplasmic Transport During Acute Ocular Hypertension in the Monkey." Investigative Ophthalmology & Visual Science, vol. 16, no. 5, 1977, pp. 426-41.
Minckler DS, Bunt AH, Johanson GW. Orthograde and retrograde axoplasmic transport during acute ocular hypertension in the monkey. Invest Ophthalmol Vis Sci. 1977;16(5):426-41.
Minckler, D. S., Bunt, A. H., & Johanson, G. W. (1977). Orthograde and retrograde axoplasmic transport during acute ocular hypertension in the monkey. Investigative Ophthalmology & Visual Science, 16(5), 426-41.
Minckler DS, Bunt AH, Johanson GW. Orthograde and Retrograde Axoplasmic Transport During Acute Ocular Hypertension in the Monkey. Invest Ophthalmol Vis Sci. 1977;16(5):426-41. PubMed PMID: 67096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Orthograde and retrograde axoplasmic transport during acute ocular hypertension in the monkey. AU - Minckler,D S, AU - Bunt,A H, AU - Johanson,G W, PY - 1977/5/1/pubmed PY - 1977/5/1/medline PY - 1977/5/1/entrez SP - 426 EP - 41 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 16 IS - 5 N2 - Orthograde and retrograde axoplasmic transport have been studied in the optic nerve heads of 37 Macaca fascicularis eyes with normal or elevated intraocular pressure (IOP) produced by cannulation of the anterior chamber. Orthograde transport was labeled by 3H-amino acids injected intravitreally and incorporated into retinal ganglion cell proteins. Retrograde transport was studied in the same eyes by injecting horseradish peroxidase (HRP) into one or both optic tracts and dorsal lateral geniculate nuclei (dLGN). Both tracers accumulated in the lamina scleralis (LS) of eyes maintained at pressures of 25 to 150 mm. Hg for 12 to 28 hours (pressure in normal controls = 10 to 14 mm. Hg) but the HRP technique was markedly more sensitive. The degree of retrograde transport obstruction in the LS appeared to be directly proportional to both the height and the duration of elevated IOP. In one experiment, the blockades of orthograde and retrograde transport induced at 50 mm. Hg were demonstrated to be reversible. Serial reconstructions of radioautographs and peroxidase-reacted sections of the optic nerve heads demonstrated that the orthograde and retrograde transport obstructions were coincidental anatomically by light microscopy in the LS and occurred most prominently in the temporal quadrants of the nerve head. These transport obstructions occurred at moderate elevations of IOP (25 TO 50 mm. Hg) despite (1) elevated arterial PO2 levels during inhalation of 100 percent oxygen and (2) intact nerve head capillary circulation, as demonstrated by perfusion with nucleated avian erythrocytes. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/67096/Orthograde_and_retrograde_axoplasmic_transport_during_acute_ocular_hypertension_in_the_monkey_ L2 - https://iovs.arvojournals.org/article.aspx?volume=16&issue=5&page=426 DB - PRIME DP - Unbound Medicine ER -