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Steroid receptors in two types of rabbit skeletal muscle.
Int J Sports Med. 1984 Jun; 5(3):130-6.IJ

Abstract

Radiolabeled synthetic steroid hormones and a charcoal adsorption assay were used to identify cytosolic androgen, glucocorticoid, and estrogen receptors in skeletal muscle from rabbits. The presence of the receptors was verified by saturation analysis showing low-capacity, high-affinity binding for the steroid-receptor complexes, specific for each class of steroids. The concentration of androgen and estrogen receptors were of the same magnitude, whereas the corresponding value for the glucocorticoid receptor was about tenfold higher. Comparisons of fast-twitch (the gastrocnemius/plantaris complex) and slow-twitch (soleus) muscles revealed that the latter contained higher concentrations (expressed per g of tissue wet weight) of glucocorticoid and estrogen receptors, but not of androgen receptor. Expressed per mg of soluble protein, the slow-twitch muscle contained higher concentrations of all three receptors, but when related to the concentrations of all three receptors, but when related to the concentration of DNA, only the concentration of estrogen receptor was higher in the slow-twitch muscle. Different response of the two fiber types to direct action of steroid hormones can hence be expected. The fast-twitch muscle contained a higher concentration of soluble protein, whereas the slow-twitch muscle contained higher concentration of DNA, resulting in lower protein/DNA ratio, i.e., smaller "cell units," in the latter muscle.

Authors

No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

6746175

Citation

Saartok, T. "Steroid Receptors in Two Types of Rabbit Skeletal Muscle." International Journal of Sports Medicine, vol. 5, no. 3, 1984, pp. 130-6.
Saartok T. Steroid receptors in two types of rabbit skeletal muscle. Int J Sports Med. 1984;5(3):130-6.
Saartok, T. (1984). Steroid receptors in two types of rabbit skeletal muscle. International Journal of Sports Medicine, 5(3), 130-6.
Saartok T. Steroid Receptors in Two Types of Rabbit Skeletal Muscle. Int J Sports Med. 1984;5(3):130-6. PubMed PMID: 6746175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Steroid receptors in two types of rabbit skeletal muscle. A1 - Saartok,T, PY - 1984/6/1/pubmed PY - 1984/6/1/medline PY - 1984/6/1/entrez SP - 130 EP - 6 JF - International journal of sports medicine JO - Int J Sports Med VL - 5 IS - 3 N2 - Radiolabeled synthetic steroid hormones and a charcoal adsorption assay were used to identify cytosolic androgen, glucocorticoid, and estrogen receptors in skeletal muscle from rabbits. The presence of the receptors was verified by saturation analysis showing low-capacity, high-affinity binding for the steroid-receptor complexes, specific for each class of steroids. The concentration of androgen and estrogen receptors were of the same magnitude, whereas the corresponding value for the glucocorticoid receptor was about tenfold higher. Comparisons of fast-twitch (the gastrocnemius/plantaris complex) and slow-twitch (soleus) muscles revealed that the latter contained higher concentrations (expressed per g of tissue wet weight) of glucocorticoid and estrogen receptors, but not of androgen receptor. Expressed per mg of soluble protein, the slow-twitch muscle contained higher concentrations of all three receptors, but when related to the concentrations of all three receptors, but when related to the concentration of DNA, only the concentration of estrogen receptor was higher in the slow-twitch muscle. Different response of the two fiber types to direct action of steroid hormones can hence be expected. The fast-twitch muscle contained a higher concentration of soluble protein, whereas the slow-twitch muscle contained higher concentration of DNA, resulting in lower protein/DNA ratio, i.e., smaller "cell units," in the latter muscle. SN - 0172-4622 UR - https://www.unboundmedicine.com/medline/citation/6746175/Steroid_receptors_in_two_types_of_rabbit_skeletal_muscle_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2008-1025894 DB - PRIME DP - Unbound Medicine ER -