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Immunoenzymatic absorption test for serodiagnosis of paracoccidioidomycosis.
J Clin Microbiol. 1984 Jul; 20(1):103-8.JC

Abstract

A sensitive enzyme-linked immunosorbent assay is described for paracoccidioidomycosis serodiagnosis, with antigen represented by filtrates from Paracoccidioides brasiliensis cultures. Cross-reactivities were, however, observed with sera from patients with other mycoses such as histoplasmosis, lobomycosis, cryptococcosis, candidiasis, and sporotrichosis. These cross-reactions did not occur when we used as antigen Yarzabal E2 component prepared by affinity fractionation of the culture filtrates. Specific results could also be obtained with the whole filtrate antigen when sera were absorbed with Histoplasma capsulatum yeast and mycelial components. Besides a very high sensitivity (100% of the 69 cases of paracoccidioidomycosis tested), this assay showed no false-positive results for the 206 non-paracoccidioidomycosis sera studied, including those from patients with other mycotic diseases.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

6746881

Citation

Mendes-Giannini, M J., et al. "Immunoenzymatic Absorption Test for Serodiagnosis of Paracoccidioidomycosis." Journal of Clinical Microbiology, vol. 20, no. 1, 1984, pp. 103-8.
Mendes-Giannini MJ, Camargo ME, Lacaz CS, et al. Immunoenzymatic absorption test for serodiagnosis of paracoccidioidomycosis. J Clin Microbiol. 1984;20(1):103-8.
Mendes-Giannini, M. J., Camargo, M. E., Lacaz, C. S., & Ferreira, A. W. (1984). Immunoenzymatic absorption test for serodiagnosis of paracoccidioidomycosis. Journal of Clinical Microbiology, 20(1), 103-8.
Mendes-Giannini MJ, et al. Immunoenzymatic Absorption Test for Serodiagnosis of Paracoccidioidomycosis. J Clin Microbiol. 1984;20(1):103-8. PubMed PMID: 6746881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoenzymatic absorption test for serodiagnosis of paracoccidioidomycosis. AU - Mendes-Giannini,M J, AU - Camargo,M E, AU - Lacaz,C S, AU - Ferreira,A W, PY - 1984/7/1/pubmed PY - 1984/7/1/medline PY - 1984/7/1/entrez SP - 103 EP - 8 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 20 IS - 1 N2 - A sensitive enzyme-linked immunosorbent assay is described for paracoccidioidomycosis serodiagnosis, with antigen represented by filtrates from Paracoccidioides brasiliensis cultures. Cross-reactivities were, however, observed with sera from patients with other mycoses such as histoplasmosis, lobomycosis, cryptococcosis, candidiasis, and sporotrichosis. These cross-reactions did not occur when we used as antigen Yarzabal E2 component prepared by affinity fractionation of the culture filtrates. Specific results could also be obtained with the whole filtrate antigen when sera were absorbed with Histoplasma capsulatum yeast and mycelial components. Besides a very high sensitivity (100% of the 69 cases of paracoccidioidomycosis tested), this assay showed no false-positive results for the 206 non-paracoccidioidomycosis sera studied, including those from patients with other mycotic diseases. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/6746881/Immunoenzymatic_absorption_test_for_serodiagnosis_of_paracoccidioidomycosis_ L2 - https://journals.asm.org/doi/10.1128/jcm.20.1.103-108.1984?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -