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Cultural and histopathologic confirmation of canine blastomycosis diagnosed by an agar-gel immunodiffusion test.
Am J Vet Res. 1980 Aug; 41(8):1263-5.AJ

Abstract

Sixteen sera from 17 dogs with blastomycosis produced a precipitin band identical with the diagnostically significant precipitin A band formed by a Blastomyces dermatitidis reference antiserum and a soluble B dermatitidis yeast-form antigen in the agar-gel immunodiffusion test (94% sensitivity). The other serum from a dog with histopathologic demonstration of B dermatitidis in pulmonic tissues produced an unrelated precipitin band. Sixteen of the 17 diagnoses made by the detection of precipitin A were confirmed by isolation and culture of B dermatitidis or by histopathologic demonstration of the pathogen. Three cases were confirmed by cultural isolation only, 10 by histopathologic demonstration only, and 3 by both. In three other dogs given amphotericin B, there were demonstrable changes in serum precipitin A reactions.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

6778271

Citation

Phillips, W E., and L Kaufman. "Cultural and Histopathologic Confirmation of Canine Blastomycosis Diagnosed By an Agar-gel Immunodiffusion Test." American Journal of Veterinary Research, vol. 41, no. 8, 1980, pp. 1263-5.
Phillips WE, Kaufman L. Cultural and histopathologic confirmation of canine blastomycosis diagnosed by an agar-gel immunodiffusion test. Am J Vet Res. 1980;41(8):1263-5.
Phillips, W. E., & Kaufman, L. (1980). Cultural and histopathologic confirmation of canine blastomycosis diagnosed by an agar-gel immunodiffusion test. American Journal of Veterinary Research, 41(8), 1263-5.
Phillips WE, Kaufman L. Cultural and Histopathologic Confirmation of Canine Blastomycosis Diagnosed By an Agar-gel Immunodiffusion Test. Am J Vet Res. 1980;41(8):1263-5. PubMed PMID: 6778271.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cultural and histopathologic confirmation of canine blastomycosis diagnosed by an agar-gel immunodiffusion test. AU - Phillips,W E,Jr AU - Kaufman,L, PY - 1980/8/1/pubmed PY - 2001/3/28/medline PY - 1980/8/1/entrez SP - 1263 EP - 5 JF - American journal of veterinary research JO - Am J Vet Res VL - 41 IS - 8 N2 - Sixteen sera from 17 dogs with blastomycosis produced a precipitin band identical with the diagnostically significant precipitin A band formed by a Blastomyces dermatitidis reference antiserum and a soluble B dermatitidis yeast-form antigen in the agar-gel immunodiffusion test (94% sensitivity). The other serum from a dog with histopathologic demonstration of B dermatitidis in pulmonic tissues produced an unrelated precipitin band. Sixteen of the 17 diagnoses made by the detection of precipitin A were confirmed by isolation and culture of B dermatitidis or by histopathologic demonstration of the pathogen. Three cases were confirmed by cultural isolation only, 10 by histopathologic demonstration only, and 3 by both. In three other dogs given amphotericin B, there were demonstrable changes in serum precipitin A reactions. SN - 0002-9645 UR - https://www.unboundmedicine.com/medline/citation/6778271/Cultural_and_histopathologic_confirmation_of_canine_blastomycosis_diagnosed_by_an_agar_gel_immunodiffusion_test_ DB - PRIME DP - Unbound Medicine ER -
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