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Validity of transcobalamin II-based radioassay for the determination of serum vitamin B12 concentrations.
Ann Clin Biochem. 1980 Nov; 17(6):287-92.AC

Abstract

A valid radioassay for the estimation of serum vitamin B12 in the presence of naturally occurring vitamin B12 (= cobalamin) analogues can be operated if serum transcobalamin II (TC II) is used as the binding protein. Serum samples that gave diagnostically discrepant results when their vitamin B12 content was analysed (i) by a commercial radioassay known to be susceptible to interference from cobalamin analogues, and (ii) by microbiological assay, were further analysed by an alternative radioassay which uses the transcobalamins (principally TC II) of diluted normal serum as the assay binding protein. Concordance between the results from microbiological assay and the TC II-based radioassay was found in all cases. In an extended study over a three-year period, all routine serum samples sent for vitamin B12 analysis that had a vitamin B12 content of less than 320 ng/l by the TC II-based radioassay (reference range 200-850 ng/l) were reanalysed using an established microbiological method. Over 1000 samples were thus analysed. The data are presented to demonstrate the validity of the TC II-based radioassay results in this group of patients, serum samples from which are most likely to produce diagnostically erroneous vitamin B12 results when analysed by a radioassay that is less specific for cobalamins.

Authors

Paltridge G
No affiliation info available
Rudzki Z
No affiliation info available
Ryall RG
No affiliation info available

MeSH

Biological AssayBlood ProteinsCobalt RadioisotopesEuglena gracilisHumansReagent Kits, DiagnosticTranscobalaminsVitamin B 12Vitamin B 12 Deficiency

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

6782932

Citation

Paltridge, G, et al. "Validity of Transcobalamin II-based Radioassay for the Determination of Serum Vitamin B12 Concentrations." Annals of Clinical Biochemistry, vol. 17, no. 6, 1980, pp. 287-92.
Paltridge G, Rudzki Z, Ryall RG. Validity of transcobalamin II-based radioassay for the determination of serum vitamin B12 concentrations. Ann Clin Biochem. 1980;17(6):287-92.
Paltridge, G., Rudzki, Z., & Ryall, R. G. (1980). Validity of transcobalamin II-based radioassay for the determination of serum vitamin B12 concentrations. Annals of Clinical Biochemistry, 17(6), 287-92.
Paltridge G, Rudzki Z, Ryall RG. Validity of Transcobalamin II-based Radioassay for the Determination of Serum Vitamin B12 Concentrations. Ann Clin Biochem. 1980;17(6):287-92. PubMed PMID: 6782932.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validity of transcobalamin II-based radioassay for the determination of serum vitamin B12 concentrations. AU - Paltridge,G, AU - Rudzki,Z, AU - Ryall,R G, PY - 1980/11/1/pubmed PY - 1980/11/1/medline PY - 1980/11/1/entrez SP - 287 EP - 92 JF - Annals of clinical biochemistry JO - Ann Clin Biochem VL - 17 IS - 6 N2 - A valid radioassay for the estimation of serum vitamin B12 in the presence of naturally occurring vitamin B12 (= cobalamin) analogues can be operated if serum transcobalamin II (TC II) is used as the binding protein. Serum samples that gave diagnostically discrepant results when their vitamin B12 content was analysed (i) by a commercial radioassay known to be susceptible to interference from cobalamin analogues, and (ii) by microbiological assay, were further analysed by an alternative radioassay which uses the transcobalamins (principally TC II) of diluted normal serum as the assay binding protein. Concordance between the results from microbiological assay and the TC II-based radioassay was found in all cases. In an extended study over a three-year period, all routine serum samples sent for vitamin B12 analysis that had a vitamin B12 content of less than 320 ng/l by the TC II-based radioassay (reference range 200-850 ng/l) were reanalysed using an established microbiological method. Over 1000 samples were thus analysed. The data are presented to demonstrate the validity of the TC II-based radioassay results in this group of patients, serum samples from which are most likely to produce diagnostically erroneous vitamin B12 results when analysed by a radioassay that is less specific for cobalamins. SN - 0004-5632 UR - https://www.unboundmedicine.com/medline/citation/6782932/Validity_of_transcobalamin_II_based_radioassay_for_the_determination_of_serum_vitamin_B12_concentrations_ DB - PRIME DP - Unbound Medicine ER -