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Heterogeneity of human monocyte subsets in the promotion of B cell colonies and the role of interleukin 1.
J Immunol. 1982 Aug; 129(2):455-60.JI

Abstract

The abilities of human peripheral blood mononuclear-phagocyte (M phi) subpopulations and of interleukin 1 (IL 1) to support human B cell colony formation in semisolid cultures stimulated by staph protein A were analyzed. Human M phi subsets enriched for complement receptors (CR) effectively functioned as accessory cells supporting colony growth, whereas the responses obtained with CR-depleted M phi were 4.6-fold less. In experiments analyzing IL 1 production, CR-enriched M phi secreted four to 12 fold greater amounts of basal and stimulated IL 1 than CR-depleted M phi. Also, the addition of IL 1 to CR-depleted M phi resulted in a fourfold increase of colony numbers. The responses of cultures containing CR-depleted M phi plus IL 1, however, remained 30% less than those observed for cultures supplemented with CR-enriched M phi. Other studies showed that IL 1 was unable to substitute for M phi; the responses of cultures containing IL 1 and B cells were reduced 10-fold compared to cultures supplemented with autologous M phi. These findings indicate that human M phi subsets exist that differ in their ability to function as accessory cells. Although IL 1 can collaborate with certain M phi subsets to restore their accessory cell function, it cannot replace intact M phi. Thus, it is possible that other monokines or lymphokines play a role in M phi accessory cell function.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

6806370

Citation

Whisler, R L., et al. "Heterogeneity of Human Monocyte Subsets in the Promotion of B Cell Colonies and the Role of Interleukin 1." Journal of Immunology (Baltimore, Md. : 1950), vol. 129, no. 2, 1982, pp. 455-60.
Whisler RL, Newhouse YG, Lachman LB. Heterogeneity of human monocyte subsets in the promotion of B cell colonies and the role of interleukin 1. J Immunol. 1982;129(2):455-60.
Whisler, R. L., Newhouse, Y. G., & Lachman, L. B. (1982). Heterogeneity of human monocyte subsets in the promotion of B cell colonies and the role of interleukin 1. Journal of Immunology (Baltimore, Md. : 1950), 129(2), 455-60.
Whisler RL, Newhouse YG, Lachman LB. Heterogeneity of Human Monocyte Subsets in the Promotion of B Cell Colonies and the Role of Interleukin 1. J Immunol. 1982;129(2):455-60. PubMed PMID: 6806370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Heterogeneity of human monocyte subsets in the promotion of B cell colonies and the role of interleukin 1. AU - Whisler,R L, AU - Newhouse,Y G, AU - Lachman,L B, PY - 1982/8/1/pubmed PY - 1982/8/1/medline PY - 1982/8/1/entrez SP - 455 EP - 60 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 129 IS - 2 N2 - The abilities of human peripheral blood mononuclear-phagocyte (M phi) subpopulations and of interleukin 1 (IL 1) to support human B cell colony formation in semisolid cultures stimulated by staph protein A were analyzed. Human M phi subsets enriched for complement receptors (CR) effectively functioned as accessory cells supporting colony growth, whereas the responses obtained with CR-depleted M phi were 4.6-fold less. In experiments analyzing IL 1 production, CR-enriched M phi secreted four to 12 fold greater amounts of basal and stimulated IL 1 than CR-depleted M phi. Also, the addition of IL 1 to CR-depleted M phi resulted in a fourfold increase of colony numbers. The responses of cultures containing CR-depleted M phi plus IL 1, however, remained 30% less than those observed for cultures supplemented with CR-enriched M phi. Other studies showed that IL 1 was unable to substitute for M phi; the responses of cultures containing IL 1 and B cells were reduced 10-fold compared to cultures supplemented with autologous M phi. These findings indicate that human M phi subsets exist that differ in their ability to function as accessory cells. Although IL 1 can collaborate with certain M phi subsets to restore their accessory cell function, it cannot replace intact M phi. Thus, it is possible that other monokines or lymphokines play a role in M phi accessory cell function. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/6806370/Heterogeneity_of_human_monocyte_subsets_in_the_promotion_of_B_cell_colonies_and_the_role_of_interleukin_1_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=6806370 DB - PRIME DP - Unbound Medicine ER -